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首页> 外文期刊>Acta Horticulturae >Using Proteomics and Genomics to Search for Low Temperature Virulence Factors in Ralstonia solanacearum
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Using Proteomics and Genomics to Search for Low Temperature Virulence Factors in Ralstonia solanacearum

机译：使用蛋白质组学和基因组学来寻找青枯雷尔氏菌的低温毒力因子

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Ralstonia solanacearum is the causal agent of bacterial wilt of solanaceous plants. In the USA the disease is of economic importance in potato, tomato and tobacco crops. Strains of the Race 3 Biovar 2 (R3B2) can infect tomato and potato at low temperatures and are not established in North America, thus posing a threat to its agriculture. In previous works we identified strains of R1B1 sequevar 4 that are pathogenic on tomato at low temperatures, and are endemic in Florida. Because of the potential threat to the tomato industry we sought to identify potential virulence factors at low temperatures. Using a proteomics approach we identified a core set of 31 proteins that are produced differentially at 30 and 18°C. These proteins are present in both cool virulent and non-pathogenic strains at 18°C; however, their abundance is dependent on temperature. Preliminary virulence tests of a cool virulent R1B1 strain (P673) suggest that virulence of a mutant impaired in twitching motility is dependent on temperature. Currently, we are sequencing the genome of P673. Using comparative genomics of phylogenetically closely related strains we are planning to search for genetic factors that are present in cool virulent strains and absent in non-pathogenic ones atlow temperatures. The combined results will provide new insights into the genetic factors and mechanisms of virulence at low temperatures.
机译：青枯雷尔氏菌是茄科植物枯萎病的病原体。在美国，该病在马铃薯，番茄和烟草作物中具有重要的经济意义。第3种族Biovar 2（R3B2）菌株可以在低温下感染番茄和马铃薯，并且在北美尚未建立，因此对其农业构成威胁。在以前的工作中，我们确定了R1B1 sequevar 4菌株，这些菌株在低温下对番茄致病，并在佛罗里达州流行。由于对番茄行业的潜在威胁，我们试图确定低温下的潜在毒力因子。使用蛋白质组学方法，我们确定了31种蛋白质的核心集，这些蛋白质在30和18°C下会产生差异。这些蛋白在18°C的冷毒株和非致病株中均存在。但是，它们的丰度取决于温度。酷毒R1B1毒株（P673）的初步毒力测试表明，抽动运动受损的突变体的毒力取决于温度。目前，我们正在对P673的基因组进行测序。我们计划使用系统发育上密切相关的菌株的比较基因组学，以寻找存在于冷毒力菌株中且在低温下非致病性菌株中不存在的遗传因素。结合的结果将提供对低温的遗传因素和毒力机制的新见解。

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