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首页> 外文期刊>Acta Horticulturae >Exogenous Polyamines and Ethylene on Aechmea distichantha L. (Bromeliaceae) Micropropagation
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Exogenous Polyamines and Ethylene on Aechmea distichantha L. (Bromeliaceae) Micropropagation

机译:Aechmea distichantha L.(Bromeliaceae)微繁殖上的外源多胺和乙烯

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摘要

The aim of the research was to study the effects of exogenous polyamines putrescine, spermidine and spermine and ethylene on in vitro morphogenesis of Aechmea distichantha {Bromeliaceae). The plants obtained in vitro by shoots division were transferred to MS growth regulators free, MS + 10 fimol Put, MS + 10 fimol Spd, MS + 10 nmol Spm, MS + 10 mg/L ethylene and MS + 20 mg/L ethylene. Plants were harvest after 0, 15, 30, 45 and 60 days of culture. Number of shoots and roots and endogenous concentrations of polyamines Put, Spd and Spm, protein and peroxidase activity in leaves were evaluated. Spd stimulated shoots formation on A. distichantha. All treatments had deleterious effect on rhizogenesis. Plants treated with polyamines had higher proteins levels when compared to ethylene indicating the growing and development of explants. Ethylene treatment had no effect on polyamines levels. Thus, the biosynthetic routes of ethylene and polyamines may not compete for the common precursor S-adenosylmethionine.
机译:该研究的目的是研究外源多胺腐胺,亚精胺,亚精胺和乙烯对Aechmea distichantha(Bromeliaceae)体外形态发生的影响。通过芽分裂体外获得的植物被转移至无MS生长调节剂的,MS + 10fimol Put,MS + 10fimol Spd,MS + 10nmol Spm,MS + 10mg / L乙烯和MS + 20mg / L乙烯。培养0、15、30、45和60天后收获植物。评估了芽和根的数目以及叶片中多胺Put,Spm和Spm的内源浓度,蛋白质和过氧化物酶的活性。 Spd刺激了A. distichantha上的新芽形成。所有处理均对根际产生有害作用。与乙烯相比,用多胺处理的植物蛋白质含量更高,表明外植体的生长和发育。乙烯处理对多胺水平没有影响。因此,乙烯和多胺的生物合成途径可能无法与常见的前体S-腺苷甲硫氨酸竞争。

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