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首页> 外文期刊>Acta Horticulturae >In Vitro Propagation and Rooting of Pear Cultivars
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In Vitro Propagation and Rooting of Pear Cultivars

机译：梨品种的离体繁殖和生根

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The use of in vitro culture approaches constitutes an important tool for multiplication of selected pear cultivars. The goal of this study was to develop a method for rapid in vitro shoot multiplication of pear cultivars (‘Beurre Alexandre Lucas’, ‘Milada’ and ‘Rote Williams’). These cultivars are important or prospective in the Czech pear-growing industry. For the shoot multiplication, six MS media containing 1, 2 and 4 mg L ' BAP, 0.5 and 1 mg L ‘ TDZ or 10 mg l ’ 2iP were tested. Selectedpear cultivars were successfully established in vitro using shoot tips from grafted plants grown in a glasshouse and 0.15% HgCh as a sterilization solution. The number of newly formed shoots varied with the cultivar and concentration of plant growth regulator. The relatively high levels of cytokinin BAP (4 mg L'*) produced a high number of shoots in our experiments with pear cultivars ‘Beurre Alexandre Lucas’ (5.0±0.2) and ‘Milada’ (3.8±0.2). In contrary, the lowest multiplication rates were obtained on MS medium with 10 mg L~(-1) 2iP. Pear cultivar ‘Rote Williams’ produced shoots with abnormally narrow leaves on all media with BAP and TDZ. Omission of cytokinins and exposure to a high auxin concentration1mg L~(-1) NAA was effective for root induction in the case of all tested cultivars. Successful in vitro propagation, which can be carried during the dormant season, could provide an alternative means to produce disease-free stock material or stock material of particular cultivars for sanitation from viruses (chemotherapy, thermotherapy).
机译：体外培养方法的使用构成了繁殖所选梨品种的重要工具。这项研究的目的是开发一种梨品种（“ Beurre Alexandre Lucas”，“ Milada”和“ Rote Williams”）快速体外繁殖的方法。这些品种在捷克梨种植业中很重要或有前景。对于芽繁殖，测试了六种MS培养基，分别含有1、2和4 mg L'BAP，0.5和1 mg L’TDZ或10 mg l’2iP。使用在温室中生长的嫁接植物的芽尖和0.15％HgCh作为灭菌溶液，成功地在体外建立了选定的梨品种。新形成的芽的数量随品种和植物生长调节剂的浓度而变化。在我们的梨品种“ Beurre Alexandre Lucas”（5.0±0.2）和“ Milada”（3.8±0.2）的实验中，相对较高水平的细胞分裂素BAP（4 mg L'*）产生了大量芽。相反，在具有10 mg L〜（-1）2iP的MS培养基上获得最低的繁殖率。在BAP和TDZ的所有培养基上，梨品种'Rote Williams'的芽均具有异常狭窄的叶片。在所有受试品种中，省略细胞分裂素并暴露于高植物生长素浓度1mg L〜（-1）NAA均能有效诱导根。可以在休眠季节进行的成功的体外繁殖，可以提供另一种方法来生产无病的库存材料或特定品种的库存材料，以从病毒中进行卫生消毒（化学疗法，热疗法）。

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