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首页> 外文期刊>Acta Horticulturae >Influence of Sucrose Concentration on Photosynthetic Performance of Guzmania ‘Hilda’ In Vitro
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Influence of Sucrose Concentration on Photosynthetic Performance of Guzmania ‘Hilda’ In Vitro

机译:蔗糖浓度对古斯曼尼亚“希尔达”光合性能的影响

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摘要

Tissue culture is a commonly used technique for the propagation of bromeliads. In the presented work the influence of sucrose in the tissue culture media on the photosynthetic performance and growth of the bromeliad Guzmania ‘Hilda’ was evaluated. At the start of the in vitro phase sucrose plays a pivotal role in providing carbohydrate sources but concomitantly it might also constitute a trigger for feedback inhibition for photosynthesis. Therefore the application of sucrose was studied in more detail by exposing the in vitro plants to different exogenous sucrose concentrations (5, 12.5, 25 and 40 g/L) in the rooting medium (final stage). The amount of sucrose showed a positive relationship with both biomass formation and dawn CO2 concentrations in the culture containers. The CO2 uptake capacity was comparable for plants grown on 12.5, 25 and 40 g/L sucrose, but significantly lower for plants grown on 5 g/L sucrose during rooting stage. The initial sucrose concentration was already depleted after4 weeks in all treatments except for the highest initial concentration, where complete eradication took8weeks. As such, sucrose is unable to interfere with in vitro photosynthetic performance. However, high concentrations of hexoses (glucose and fructose) were present in the medium but did not suppress photosynthesis as a higher CO_2 uptake capacity and biomass formation did occur on initial sucrose enriched culture media.
机译:组织培养是凤梨科植物繁殖的常用技术。在提出的工作中,评估了蔗糖在组织培养基中对凤梨科古斯曼“希尔达”的光合性能和生长的影响。在体外阶段开始时,蔗糖在提供碳水化合物来源方面起着关键作用,但同时也可能构成光合作用反馈抑制的触发因素。因此,通过在生根培养基(最终阶段)中使体外植物暴露于不同的外源蔗糖浓度(5、12.5、25和40 g / L),对蔗糖的应用进行了更详细的研究。蔗糖的量与培养容器中的生物量形成和黎明的CO2浓度呈正相关。在生根阶段,以12.5、25和40 g / L蔗糖生长的植物的CO2吸收能力相当,但是对于以5 g / L蔗糖生长的植物,二氧化碳的吸收能力却相当低。在所有处理中,最初的蔗糖浓度已经在4周后耗尽,除了最高的初始浓度(完全根除需要8周)外。因此,蔗糖不能干扰体外光合作用。但是,培养基中存在高浓度的己糖(葡萄糖和果糖),但并未抑制光合作用,因为较高的CO_2吸收能力和在富含蔗糖的初始培养基上确实发生了生物量形成。

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