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首页> 外文期刊>Acta Horticulturae >An Effective Embryogenesis Procedure from Anther Culture in Loquat
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An Effective Embryogenesis Procedure from Anther Culture in Loquat

机译：Lo花药培养的有效胚胎发生程序

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摘要

The objective of this study was to establish a highly effective embryogenesis procedure in anther culture of loquat (Eriobotrya japonica Lindl. 'Dawuxing'). The results showed correlations between diameters of flower bud and microspore developmental stages. When the transverse and vertical diameters of flower buds were 4.68 and 4.52 mm, respectively, microspores were at the late-uninucleate developmental stage, and were suitable for inducing embryos. Two days of cold treatment at 4°C under darknesswere more favorable than the control, resulting in 69.89% of callus formation. The most suitable medium for induction of callus from anthers was MS medium supplemented with 0.5 mg/L 2,4-D and 2.0 mg/L 6-BA, in which calli were induced at a frequency of 78.3%. The optimal induction of anther-derived embryos was achieved on MS medium supplemented with 0.05 mg/L ZT, 0.01 mg/L NAA and 0.05 mg/L IBA, in which the rate of embryo induction was 25.7%. The most suitable medium for anther-derived embryo proliferation was MS supplemented with 0.05 mg/L ZT, 0.02 mg/L NAA and 0.02 mg/L IBA, in which the proliferation coefficient was 5.8.
机译：这项研究的目的是在in的花药培养中建立一种高效的胚胎发生程序（Eriobotrya japonica Lindl。'Dawuxing'）。结果表明，花芽直径与小孢子发育阶段之间具有相关性。当花芽的横向和垂直直径分别为4.68和4.52 mm时，小孢子处于单核发育后期，适合诱导胚芽。在黑暗中于4°C下进行两天的冷处理比对照组更有利，导致愈伤组织形成的69.89％。最适合从花药中诱导愈伤组织的培养基是补充有0.5 mg / L 2,4-D和2.0 mg / L 6-BA的MS培养基，其中愈伤组织的诱导频率为78.3％。在补充有0.05 mg / L ZT，0.01 mg / L NAA和0.05 mg / L IBA的MS培养基上，最佳诱导了花药来源的胚胎，其中胚胎诱导率为25.7％。最适合花药胚胎增殖的培养基是MS，添加0.05 mg / L ZT，0.02 mg / L NAA和0.02 mg / L IBA，其增殖系数为5.8。

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