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首页> 外文期刊>Acta Horticulturae >Micropropagation of Quercus robur: Explant Sources and Cultural Conditions Affect In Vitro Responses Differently
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Micropropagation of Quercus robur: Explant Sources and Cultural Conditions Affect In Vitro Responses Differently

机译：罗伯栎的微繁殖：外植体来源和培养条件对体外反应的影响不同。

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摘要

In this study results regarding in vitro propagation and conservation of oali germplasm are presented. Fruits and stem cuttings of Q. robur were collected from mature trees. Acorns were put in mould and the seedlings used as source of explants, nodalsegments with apical and axillary buds. Nodal segments from stem cuttings were also used. Embryos isolated from acorns were cultured to obtain in vitro growing seedlings: nodal segments from these seedlings were considered a third type of explant. We also tested the same type of explant from shoots at the second cycle of micropropagation. All the explants were placed on Woody Plant Medium with BA 0.5 mg/L. Buds from in vitro seedlings showed the highest percentage of shoot regeneration. The highest number of shoots per productive explant was observed in buds from seedlings grown in mould. With regard to former position of the buds on the stem we observed a prevalent callus proliferation in buds from basal position. Callus occurrence seemed to negatively influence the following shoot development. Afterwards, shoots cultured on Woody Plant Medium containing different auxins, with or without activated carbon, exhibited a good rooting ability. The addition of activated carbon to culture medium seemed to prevent shoots browning. Rooted plantlets were acclimatized after 2 and 11 months. No difference in terms of survival was observed.
机译：在这项研究中，提出了有关燕麦种质体外繁殖和保存的结果。罗伯Q. robur的果实和茎条均采自成熟树木。将橡子放入霉菌中，将幼苗用作外植体，带有顶端和腋芽的节节的来源。还使用了来自茎插的节节。培养从橡子中分离出的胚以获得体外生长的幼苗：这些幼苗的节节被认为是第三种外植体。在微繁繁殖的第二个周期中，我们还从芽中测试了相同类型的外植体。将所有外植体置于BA 0.5 mg / L的木本植物培养基上。体外幼苗的芽显示出最高的芽再生百分比。在模具中生长的幼苗的芽中，观察到每个生产性外植体的芽数最高。关于芽在茎上的先前位置，我们观察到芽在基底位置上普遍存在愈伤组织增殖。愈伤组织的出现似乎对随后的嫩芽发育产生负面影响。此后，在含有不同生长素，有或没有活性炭的木本植物培养基上培养的芽表现出良好的生根能力。向培养基中添加活性炭似乎可以防止芽褐变。在2和11个月后使生根的幼苗适应环境。在存活率方面没有观察到差异。

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