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首页> 外文期刊>Acta Horticulturae >Effect of Osmotic Regulators on In Vitro Conservation of Brazilian Ginseng, Potato and Cassava Germplasms
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Effect of Osmotic Regulators on In Vitro Conservation of Brazilian Ginseng, Potato and Cassava Germplasms

机译：渗透调节剂对巴西人参，马铃薯和木薯种质体外保存的影响

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摘要

Slow growth in vitro conservation is widely used in the conservation of germplasm of various species. Changes in the chemical conditions of the culture medium, using e.g., osmotic regulators, has been used as a strategy for reducing the in vitro plantmetabolism and prolonging the cultivation period. The objective of this study is to evaluate the use of osmotic regulators on the in vitro conservation of Brazilian ginseng {Pfaffla glomemta), potato {Solanum tuberosum) and cassava (Manihot esculenta) germplasms for reducing the in vitro plant growth and prolonging the cultivation period. Nodal segments from in vitro grown plantlets were cultured on MD medium (modified MS salts and vitamins, 0.02 mg/L BAP, 0.01 mg/L NAA, 0.1 mg/L GA3) for cassava or MSO medium (MS medium without growth regulator) for Brazilian ginseng and potato, added sorbitol (0, 20, 40, or 60g/L) or mannitol (0 to 20 g/L) as osmotic regulator, maintained for 9 months at 20°C and 45 ^mol m' s ' photosynthetic photon flux density oflight intensity with a 12-h photoperiod. Effects of the osmotic regulators were different depending on the culture period and species. The sorbitol at concentrations of 20 and 40 g/L delayed the shoot growth and could be used for the in vitro conservation of potato and Brazilian ginseng germplasms. For the conservation of cassava germplasms, mannitol at a concentration of 20 g/L was suitable as an osmotic regulator.
机译：体外保存缓慢生长广泛用于各种物种的种质保存。使用例如渗透调节剂的培养基化学条件的变化已被用作减少体外植物代谢并延长培养时间的策略。这项研究的目的是评估渗透调节剂在巴西人参（Pfaffla glomemta），马铃薯（Solanum tuberosum）和木薯（Manihot esculenta）种质的体外保存中的用途，以减少体外植物的生长并延长栽培时间。 。将用于木薯的MD培养基（改良的MS盐和维生素，0.02 mg / L BAP，0.01 mg / L NAA，0.1 mg / L GA3）培养用于体外生长苗的节点节段，以用于木薯培养。巴西人参和马铃薯，添加山梨糖醇（0、20、40或60g / L）或甘露醇（0至20 g / L）作为渗透调节剂，在20°C和45 mol / mol的光合作用下保持9个月光子通量密度光照强度为12 h的光周期。渗透调节剂的作用因培养时期和种类而异。浓度为20和40 g / L的山梨糖醇延迟了芽的生长，可用于马铃薯和巴西人参种质的体外保存。为了保护木薯种质，浓度为20 g / L的甘露醇适合作为渗透调节剂。

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