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Towards a genome-wide association (GWA) mapping approach in globe artichoke

机译:朝鲜蓟的全基因组关联(GWA)定位方法

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摘要

Here we present the first insights on a genome-wide association approach in globe artichoke, based on an association panel composed of 111 varietal types coming from the living collection maintained at AGRIS (Sardinia, Italy). The collection has beeninvestigated through two complementary molecular approaches: (i) microsatellite (SSRs) genotyping, using 26 loci uniformly distributed throughout the genome to investigate the population structure of the core germplasm set; and (ii) genotyping by sequencing, using a two-enzymes RADseq (restriction-site associated DNA sequencing) technology, as a basis for an association mapping approach.
机译:在这里,我们将基于朝鲜蓟(AGRIS)(意大利撒丁岛)保存的111种不同品种组成的关联小组,提出一种针对朝鲜蓟全基因组关联方法的初步见解。已通过两种互补的分子方法对收集物进行了研究:(i)微卫星(SSRs)基因分型,使用在整个基因组中均匀分布的26个基因座来研究核心种质集的种群结构; (ii)使用两种酶RADseq(限制性位点相关的DNA测序)技术通过测序进行基因分型,以此作为关联作图方法的基础。

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