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首页> 外文期刊>ACS applied materials & interfaces >Effects of Zero-Length and Non-Zero-Length Cross-Linking Reagents on the Optical Spectral Properties and Structures of Collagen Hydrogels
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Effects of Zero-Length and Non-Zero-Length Cross-Linking Reagents on the Optical Spectral Properties and Structures of Collagen Hydrogels

机译：零长度和非零长度交联剂对胶原水凝胶的光谱性质和结构的影响

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摘要

We compared the effects of zero-length cross-linkers 1-ethyl-3 (3dimethylaminopropyl) carbodiimide (EDC) and nonzero-length cross-linkers glycolaldehyde and glyceraldehyde on the optical and structural properties of three-dimensional (3D) collagen hydrogels. We evaluated these effects by multiphoton microscopy (MPM) that combined two-photon fluorescence (TPF) and second harmonic generation (SHG) contrasts and transmission electron microscopy (TEM). The collagen hydrogels were incubated separately with the above-mentioned reagents present at theconcentration of 0.1 M. The incubation with glycolaldehyde and glyceraldehyde induced strong autorluorescence within the gels. We followed the formation of fluorescence with TPF signals in situ and in real time as well as characterized the micro- and nanostructures within cross-linked hydrogels by examining SHG and TEM images respectively. As detected in the SHG images, glycolaldehyde- and glyceraldehyde-modified S-10 μm "fiberlike" collagen structures to longer, 20 μm and more, aggregated strands while EDC had minimal effect on the microstructure. TEM revealed that glycolaldehyde and glyceraldehyde either completely eliminated collagen's characteristic native fibrillar striations or generated uncharacteristic fibrils with extensions. EDC preserved the native striation patterns, decreased the fibril diameters and effectively homogenized the fibrils within hydrogels assembled at 1.8-4.68 g/L collagen concentrations and 37 °C. Our findings provide a clear understanding on how different cross-linking reagents have very different effects on the collagen hydrogels. This understanding is critical for advancing tissue engineering and wound healing applications.
机译：我们比较了零长度交联剂1-乙基-3（3二甲基氨基丙基）碳二亚胺（EDC）和非零长度交联剂乙二醇醛和甘油醛对三维（3D）胶原水凝胶的光学和结构性质的影响。我们通过结合双光子荧光（TPF）和二次谐波产生（SHG）对比的多光子显微镜（MPM）以及透射电子显微镜（TEM）评估了这些影响。将胶原蛋白水凝胶与浓度为0.1 M的上述试剂分别孵育。与乙醇醛和甘油醛的孵育会引起凝胶内强烈的自发荧光。我们跟踪了荧光原位和实时TPF信号的形成，并通过分别检查SHG和TEM图像表征了交联水凝胶中的微观和纳米结构。正如在SHG图像中检测到的那样，乙醇醛和甘油醛修饰的S-10μm“纤维状”胶原蛋白结构更长，20μm甚至更多，聚集了股线，而EDC对微观结构的影响最小。 TEM显示，乙醇醛和甘油醛完全消除了胶原蛋白特有的天然原纤维条纹，或生成了具有延伸特征的原纤维。 EDC保留了天然的条纹图案，减小了原纤维直径，并有效地均化了胶原蛋白浓度为1.8-4.68 g / L和37°C的水凝胶中的原纤维。我们的发现对不同的交联剂对胶原蛋白水凝胶的影响有明显的了解。这种理解对于推进组织工程和伤口愈合应用至关重要。

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