• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>ACS applied materials & interfaces >Ratiometric Nanothermometer Based on Rhodamine Dye-Incorporated F127-Melamine-Formaldehyde Polymer Nanoparticle: Preparation, Characterization, Wide-Range Temperature Sensing, and Precise Intracellular Thermometry
【24h】

Ratiometric Nanothermometer Based on Rhodamine Dye-Incorporated F127-Melamine-Formaldehyde Polymer Nanoparticle: Preparation, Characterization, Wide-Range Temperature Sensing, and Precise Intracellular Thermometry

机译：基于罗丹明染料掺入的F127-三聚氰胺-甲醛聚合物纳米粒子的比例式纳米温度计：制备，表征，宽范围的温度传感和精确的细胞内测温。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A series of fluorescent nanothermometers (FTs) was prepared with Rhodamine dye-incorporated Pluronic F-127-melamine-formaldehyde composite polymer nanoparticles (R-F127-MF NPs). The highly soluble Rhodamine dye molecules were bound with Pluronic F127 micelles and subsequently incorporated in the cross-linked MF resin NPs during high-temperature cross-link treatment. The morphology and chemical structure of R-F127-MF NPs were characterized with dynamic light scattering, electron microscopy, and Fourier-transform infrared (FTIR) spectra. Fluorescence properties and thermoresponsivities were analyzed using fluorescence spectra. R-F127-MF NPs are found to be monodispersed, presenting a size range of 88105 nm, and have bright fluorescence and high stability in severe treatments such as autoclave sterilization and lyophilization. By simultaneously incorporating Rhodamine B and Rhodamine 110 (as reference) dyes at a doping ratio of 1:400 in the NPs, ratiometric FTs with a high sensibility of 7.6%.degrees C-1 and a wide temperature sensing range from -20 to 110 degrees C were obtained. The FTs exhibit good stability in solutions with varied pH, ionic strengths, and viscosities and have similar working curves in both intracellular and extracellular environments. Cellular temperature variations in Hela cells during microwave exposure were successfully monitored using the FTs, indicating their considerable potential applications in the biomedical field.
机译：用掺入罗丹明染料的Pluronic F-127-三聚氰胺-甲醛复合聚合物纳米颗粒（R-F127-MF NPs）制备了一系列荧光纳米温度计（FTs）。高溶解性罗丹明染料分子与Pluronic F127胶束结合，随后在高温交联处理过程中掺入到交联的MF树脂NP中。通过动态光散射，电子显微镜和傅立叶变换红外（FTIR）光谱对R-F127-MF NP的形态和化学结构进行了表征。使用荧光光谱分析荧光性质和热响应性。发现R-F127-MF NP是单分散的，尺寸范围为88105 nm，并且在高压灭菌和冻干等严酷处理中具有明亮的荧光和高稳定性。通过在NP中以1：400的掺杂比同时加入若丹明B和若丹明110（作为参考）染料，比例FT的灵敏度为7.6％.C-1，温度感应范围为-20至110。获得摄氏度。 FTs在具有不同pH，离子强度和粘度的溶液中显示出良好的稳定性，并且在细胞内和细胞外环境中均具有相似的工作曲线。使用FT成功监测了微波照射期间Hela细胞中的细胞温度变化，表明它们在生物医学领域中具有巨大的潜在应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号