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首页> 外文期刊>ACS applied materials & interfaces >In situ Synthesis of Fluorescent Gold Nanoclusters by Nontumorigenic Microglial Cells
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In situ Synthesis of Fluorescent Gold Nanoclusters by Nontumorigenic Microglial Cells

机译：非致瘤性小胶质细胞原位合成荧光金纳米簇

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摘要

To date, the directed in situ synthesis of fluorescent gold nanoclusters (AuNCs) has only been demonstrated in cancerous cells, with the theorized synthesis mechanism prohibiting AuNC formation in nontumorigenic cell lines. This limitation hinders potential biostabilized AuNC-based technology in healthy cells involving both chemical and mechanical analysis, such as the direct sensing of protein function and the elucidation of local mechanical environments. Thus, new synthesis strategies are required to expand the application space of AuNCs beyond cancer-focused cellular studies. In this contribution, we have developed the methodology and demonstrated the direct in situ synthesis of AuNCs in the nontumorigenic neuronal microglial line, C8B4. The as-synthesized AuNCs form in situ and are stabilized by cellular proteins. The clusters exhibit bright green fluorescence and demonstrate low (<10%) toxicity. Interestingly, elevated ROS levels were not required for the in situ formation of AuNCs, although intracellular reductants such as glutamate were required for the synthesis of AuNCs in C8B4 cells. To our knowledge, this is the first-ever demonstration of AuNC synthesis in nontumorigenic cells and, as such, it considerably expands the application space of biostabilized fluorescent AuNCs.
机译：迄今为止，荧光金纳米簇（AuNCs）的定向原位合成仅在癌细胞中得到证实，理论上的合成机制禁止在非致瘤细胞系中形成AuNC。此限制阻碍了健康细胞中涉及化学和机械分析的潜在生物稳定化基于AuNC的技术，例如直接检测蛋白质功能和阐明局部机械环境。因此，需要新的合成策略来扩大AuNCs的应用范围，使其超越针对癌症的细胞研究。在这项贡献中，我们开发了方法，并在非致瘤性神经元小胶质细胞系C8B4中证明了AuNC的直接原位合成。合成后的AuNCs原位形成并被细胞蛋白稳定。簇显示亮绿色荧光，并显示出低（<10％）毒性。有趣的是，尽管在C8B4细胞中合成AuNCs需要胞内还原剂（例如谷氨酸），但不需要AusNC的原位形成就需要增加ROS水平。据我们所知，这是非致瘤细胞中AuNC合成的首次展示，因此，它大大扩展了生物稳定的荧光AuNCs的应用空间。

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