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首页> 外文期刊>ACS applied materials & interfaces >Self-Assembled Fluorescent Bovine Serum Albumin Nanoprobes for Ratiometric pH Measurement inside Living Cells
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Self-Assembled Fluorescent Bovine Serum Albumin Nanoprobes for Ratiometric pH Measurement inside Living Cells

机译：自组装荧光牛血清白蛋白纳米探针，用于在活细胞内进行比例pH测量

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摘要

In this work, we demonstrated a new ratiometric method for the quantitative analysis of pH inside living cells. The structure of the nanosensor comprises a biofriendly fluorescent bovine serum albumin (BSA) matrix, acting as a pH probe, and pH-insensitive reference dye Alexa 594 enabling ratiometric quantitative pH measurement. The fluorescent BSA matrix was synthesized by cross-linking of the denatured BSA proteins in ethanol with glutaraldehyde. The size of the as-synthesized BSA nanoparticles can be readily manipulated from 30 to 90 nm, which exhibit decent fluorescence at the peak wavelength of 535 nm with a pH response range of 6-8. The potential of this pH sensor for intracellular pH monitoring was demonstrated inside living HeLa cells, whereby a significant change in fluorescence ratio was observed when the pH of the cell was switched from normal to acidic with anticancer drug treatment. The fast response of the nanosensor makes it a very powerful tool in monitoring the processes occurring within the cytosol.
机译：在这项工作中，我们展示了一种用于定量分析活细胞内pH的新的比例计量方法。纳米传感器的结构包括充当pH探针的生物友好型荧光牛血清白蛋白（BSA）基质和对pH不敏感的参考染料Alexa 594，可以进行比例式定量pH测量。通过将变性的BSA蛋白在乙醇中与戊二醛交联来合成荧光BSA基质。合成后的BSA纳米粒子的大小可以轻松控制在30至90 nm之间，在535 nm的峰值波长下具有6-8的pH响应，显示出不错的荧光。在活的HeLa细胞内部证明了该pH传感器用于细胞内pH监测的潜力，从而在使用抗癌药物治疗将细胞的pH从正常值切换为酸性时，可以观察到荧光比的显着变化。纳米传感器的快速响应使其成为监测细胞质内发生过程的非常强大的工具。

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