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首页> 外文期刊>American Journal of Physiology >Characteristics of L-lactic acid transport in basal membrane vesicles of human placental syncytiotrophoblast.
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Characteristics of L-lactic acid transport in basal membrane vesicles of human placental syncytiotrophoblast.

机译：人胎盘合体滋养层细胞基底膜囊泡中L-乳酸的运输特征。

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摘要

The characteristics of L-lactic acid transport across the trophoblast basal membrane were investigated and compared with those across the brush-border membrane by using membrane vesicles isolated from human placenta. The uptake of L-[(14)C]lactic acid into basal membrane vesicles was Na(+) independent, and an uphill transport was observed in the presence of a pH gradient ([H(+)](out) > [H(+)](in)). L-[(14)C]lactic acid uptake exhibited saturation kinetics with a K(m) value of 5.89 +/- 0.68 mM in the presence of a pH gradient. p-Chloromercuribenzenesulfonate and alpha-cyano-4-hydroxycinnamate inhibited the initial uptake, whereas phloretin or 4,4'-diisothiocyanostilbene-2,2'-disulfonate did not. Mono- and dicarboxylic acids suppressed the initial uptake. In conclusion, L-lactic acid transport in the basal membrane is H(+) dependent and Na(+) independent, as is also the case for the brush-border membrane transport, and its characteristics resemble those of monocarboxylic acid transporters. However, therewere several differences in the effects of inhibitors between basal and brush-border membrane vesicles, suggesting that the transporter(s) involved in L-lactic acid transport in the basal membrane of placental trophoblast may differ from those in the brush-border membrane.
机译：研究了L-乳酸在滋养层基底膜上的转运特性，并通过使用从人胎盘中分离的膜囊泡，将其与刷状边界膜上的转运特性进行了比较。 L-[（14）C]乳酸对基底膜囊泡的吸收与Na（+）无关，并且在存在pH梯度（[H（+）]（out）> [H]的情况下观察到上坡运输（+）]（in））。 L-[（14）C]乳酸的摄取在pH梯度存在下表现出饱和动力学，其K（m）值为5.89 +/- 0.68 mM。对氯叶绿素苯磺酸盐和α-氰基-4-羟基肉桂酸酯抑制了最初的摄取，而phloretin或4,4'-二异硫氰基苯乙烯2,2'-二磺酸盐则没有。一元和二元羧酸抑制了初始摄取。总之，基膜中的L-乳酸运输是H（+）依赖和Na（+）独立的，刷式边界膜运输也是如此，其特性类似于单羧酸转运蛋白。然而，基底膜和刷状边界膜囊泡的抑制剂作用存在若干差异，表明胎盘滋养层基底膜中参与L-乳酸转运的转运蛋白可能与刷状边界膜中的转运蛋白不同。 。

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