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首页> 外文期刊>American Journal of Physiology >Placental ceruloplasmin homolog is regulated by iron and copper and is implicated in iron metabolism.
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Placental ceruloplasmin homolog is regulated by iron and copper and is implicated in iron metabolism.

机译：胎盘铜蓝蛋白的同源物受铁和铜的调节，与铁的代谢有关。

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摘要

We previously reported an endogenous, membrane-bound Cu oxidase with homology to ceruloplasmin in BeWo cells, a placental choriocarcinoma cell line. In this previous study, ceruloplasmin immunoreactivity was localized to the perinuclear region and non-brush-border membranes. Here, we show that azide-sensitive oxidase activity is enriched in the same fractions, correlating subcellular localization of enzyme activity with ceruloplasmin immunoreactivity. Expression of the placental Cu oxidase is inversely proportional to Fe status and directly proportional to Cu status at enzyme and protein levels. To identify a role for the Cu oxidase, cells were exposed to (59)Fe-transferrin for 18 h in an environment of 20% O(2) or 5% O(2). At 5% O(2), Cu-deficient cells retain significantly more (59)Fe than control cells. This excess in (59)Fe accumulation is caused by a significant decrease in (59)Fe release. These results indicate that downregulation of the placental Cu oxidase in BeWo cells impairs Fe release. Thiseffect is only apparent in an environment of limited O(2).
机译：我们之前曾报道过一种内源性，与膜结合的铜氧化酶，与BeWo细胞（胎盘绒毛膜癌细胞系）中的铜蓝蛋白具有同源性。在这项先前的研究中，铜蓝蛋白的免疫反应性局限于核周区域和非刷膜。在这里，我们显示了叠氮化物敏感的氧化酶活性富集在相同的分数，与酶活性与铜蓝蛋白免疫反应性相关的亚细胞定位。胎盘中铜氧化酶的表达与铁状态成反比，在酶和蛋白质水平上与铜状态成正比。为了确定铜氧化酶的作用，将细胞在20％O（2）或5％O（2）的环境中暴露于（59）Fe-转铁蛋白18小时。在5％O（2）时，缺铜细胞比对照细胞保留更多（59）Fe。 （59）Fe积累的过量是由（59）Fe释放的显着减少引起的。这些结果表明BeWo细胞中胎盘Cu氧化酶的下调削弱了Fe的释放。该效果仅在O（2）受限的环境中才明显。

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