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首页> 外文期刊>American Journal of Physiology >Suppression of mitochondria-dependent neutrophil apoptosis with thermal injury.
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Suppression of mitochondria-dependent neutrophil apoptosis with thermal injury.

机译：抑制线粒体依赖性中性粒细胞凋亡与热损伤。

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Neutrophil apoptosis is delayed under trauma and/or sepsis conditions. The mechanism for the delay has remained unclear. We hypothesize that modulation of the mitochondrial pathway of apoptosis contributes to the delay in neutrophil apoptosis with burn injury. Rats were subjected to burn injury (30% of total body surface area, 98 degrees C for 10 s) and euthanatized 24 h postinjury. Blood neutrophils from sham and burn-injured rats were isolated by Ficoll gradient centrifugation and cultured for 2 or 8 h. Neutrophil apoptosis was determined by annexin V and propidium iodide (PI) labeling and flow cytometry. Neutrophil mitochondrial morphology was assessed via histochemical staining (MitoTracker GreenFM) and confocal microscopy. Neutrophils from rats with burn injury showed a decreased level of apoptosis compared with sham rat neutrophils at both 2 and 8 h of incubation. In incubated sham rat neutrophils, mitochondria showed a change from normal "tubular" to an "aggregated" morphology. In contrast, cultured neutrophils from burn rats did not exhibit this mitochondrial morphological transition until 8 h of incubation. Compared with sham rat neutrophils, neutrophils from burn rats showed decreased levels of active caspase-9 and -3. Whereas an upregulation of Bcl-xL and a downregulation of Bax seemed to contribute to decreased apoptosis in burn rat neutrophils at 2 h of incubation, the decreased apoptosis at 8 h appeared to be associated with a decrease in Bax and increased phosphorylated Bad. These data suggest that suppression of the mitochondrial pathway plays an essential role in the delay of polymorphonuclear neutrophil apoptosis with burn injury.
机译：在创伤和/或败血症条件下，中性粒细胞凋亡被延迟。延迟的机制仍不清楚。我们假设凋亡的线粒体途径的调制有助于烧伤中性粒细胞凋亡的延迟。大鼠遭受烧伤（占全身表面积的30％，在98摄氏度下持续10 s），并在受伤后24小时实施安乐死。通过Ficoll梯度离心分离假手术和烧伤大鼠的血液中性粒细胞，并培养2或8小时。中性粒细胞凋亡是通过膜联蛋白V和碘化丙啶（PI）标记和流式细胞仪确定的。中性粒细胞线粒体形态通过组织化学染色（MitoTracker GreenFM）和共聚焦显微镜进行评估。在温育2小时和8小时时，与假大鼠中性粒细胞相比，烧伤大鼠中性粒细胞的凋亡水平降低。在温育的假大鼠中性粒细胞中，线粒体显示出从正常的“小管”形态到“聚集的”形态。相比之下，烧伤大鼠培养的嗜中性粒细胞直到孵育8小时才表现出这种线粒体形态转变。与假大鼠嗜中性粒细胞相比，烧伤大鼠嗜中性粒细胞活性caspase-9和-3水平降低。 Bcl-xL的上调和Bax的下调似乎有助于在灼烧2小时后烧伤大鼠中性粒细胞的凋亡减少，而8h的凋亡减少似乎与Bax的减少和磷酸化Bad的增加有关。这些数据表明，线粒体途径的抑制在烧伤多态核中性粒细胞凋亡的延迟中起着重要作用。

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