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首页> 外文期刊>American Journal of Physiology >Proximal airway mucous cells of ovalbumin-sensitized and -challenged Brown Norway rats accumulate the neuropeptide calcitonin gene-related peptide.
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Proximal airway mucous cells of ovalbumin-sensitized and -challenged Brown Norway rats accumulate the neuropeptide calcitonin gene-related peptide.

机译：卵清蛋白致敏和攻击的褐挪威大鼠的近端气道粘膜细胞积聚了神经肽降钙素基因相关肽。

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Mucous cell hypersecretion and increased neuropeptide production play a role in the exacerbation of symptoms associated with asthma. The source of these neuropeptides have been confined to the contributions of small afferent nerves or possibly neuroendocrine cells. We tested the hypothesis that repeated exposure to allergen would alter the sources and abundance of neuropeptides in airways. Right middle lobes from rats (8 wk old) exposed to 2.5% ovalbumin (OVA) for five episodes (30 min each) or filtered air were inflation fixed with paraformaldehyde. The lobes were dissected to expose the airway tree, permeabilized with DMSO, and incubated in antibody to rat calcitonin gene-related peptide (CGRP), followed with a fluorochrome-labeled second antibody. CGRP-positive structures were imaged via confocal microscopy. Airways were later embedded in plastic and sectioned for cell identification. In animals challenged with OVA, CGRP-positive cells, not neuroendocrine or neuronal in origin (confirmed by a lack of protein gene product 9.5 signal), were recorded along the axial path. In section, this fluorescent signal was localized to granules within epithelial cells. Alcian blue/periodic acid-Schiff staining of these same sections positively identify these cells as mucous cells. Mucous cells of animals not challenged with OVA were not positive for CGRP. We conclude that episodic allergen exposure results in the accumulation of CGRP within mucous cells, creating a new source for the release of this neuropeptide within the airway.
机译：粘液细胞分泌过多和神经肽产生增加在哮喘相关症状加重中起作用。这些神经肽的来源仅限于小传入神经或可能的神经内分泌细胞的贡献。我们检验了以下假设：反复接触变应原会改变呼吸道中神经肽的来源和数量。将大鼠（8周大）的右中叶暴露于2.5％卵清蛋白（OVA）中五次（每次30分钟）或过滤空气，用多聚甲醛固定。解剖肺叶以暴露气道树，用DMSO透化，并在针对大鼠降钙素基因相关肽（CGRP）的抗体中孵育，然后与荧光染料标记的第二抗体孵育。 CGRP阳性结构通过共聚焦显微镜成像。气道后来被嵌入塑料中，并进行切片以鉴定细胞。在受到OVA攻击的动物中，沿轴向记录了CGRP阳性细胞，而不是神经内分泌或起源于神经元（通过缺乏蛋白质基因产物9.5信号确认）。在截面中，该荧光信号定位于上皮细胞内的颗粒。这些相同部分的阿尔辛蓝/高碘酸-席夫（Schiff）染色可以肯定地将这些细胞识别为粘液细胞。未用OVA攻击的动物的粘液细胞CGRP不是阳性。我们得出的结论是，偶发性变应原暴露导致粘液细胞内CGRP的积累，从而为在气道内释放这种神经肽创造了新的来源。

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