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首页> 外文期刊>American Journal of Physiology >SLC26A7: a basolateral Cl-/HCO3- exchanger specific to intercalated cells of the outer medullary collecting duct.
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SLC26A7: a basolateral Cl-/HCO3- exchanger specific to intercalated cells of the outer medullary collecting duct.

机译：SLC26A7：基底外侧的Cl- / HCO3-交换子，专门用于髓外收集管的插层细胞。

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摘要

The outer medullary collecting duct (OMCD) plays an important role in bicarbonate reabsorption and acid-base regulation. An apical V-type H+-ATPase and a basolateral Cl-/HCO3- exchanger, located in intercalated cells of OMCD, mediate the bicarbonate reabsorption. Here we report the identification of a new basolateral Cl-/HCO3- exchanger in OMCD intercalated cells in rat kidney. Northern hybridizations demonstrated the predominant expression of this transporter, also known as SLC26A7, in the outer medulla, with lower expression levels in the inner medulla. SLC26A7 was recognized as a approximately 90-kDa band in the outer medulla by immunoblot analysis and was localized on the basolateral membrane of a subset of OMCD cells by immunocytochemical staining. No labeling was detected in the cortex. Double-immunofluorescence labeling with the aquaporin-2 and SLC26A7 antibodies or anion exchanger-1 and SLC26A7 antibodies identified the SLC26A7-expressing cells as alpha-intercalated cells. Functional studies inoocytes demonstrated that increasing the osmolality of the media (to simulate the physiological milieu in the medulla) increased the Cl-/HCO3- exchanger activity mediated via SLC26A7 by about threefold (P < 0.02 vs. normal condition). We propose that SLC26A7 is a basolateral Cl-/HCO3- exchanger in intercalated cells of the OMCD and may play an important role in bicarbonate reabsorption in medullary collecting duct.
机译：髓外收集管（OMCD）在重碳酸盐重吸收和酸碱调节中起重要作用。位于OMCD插入细胞中的顶端V型H + -ATPase和基底外侧Cl- / HCO3-交换剂介导碳酸氢盐的重吸收。在这里，我们报告在大鼠肾脏的OMCD插层细胞中新的基底外侧Cl- / HCO3-交换子的鉴定。 Northern杂交证明该转运蛋白在外延髓中的主要表达，也称为SLC26A7，在内延髓中的表达水平较低。通过免疫印迹分析将SLC26A7识别为髓质外约90 kDa的条带，并通过免疫细胞化学染色将其定位在一部分OMCD细胞的基底外侧膜上。在皮质中未检测到标记。用aquaporin-2和SLC26A7抗体或阴离子交换剂1和SLC26A7抗体进行的双免疫荧光标记将表达SLC26A7的细胞鉴定为α插入细胞。卵母细胞的功能研究表明，增加培养基的渗透压（以模拟延髓中的生理环境）可使通过SLC26A7介导的Cl- / HCO3-交换子活性增加约三倍（相对于正常情况，P <0.02）。我们建议SLC26A7是在OMCD的插层细胞中的基底外侧Cl- / HCO3-交换子，并且可能在髓样收集管中的碳酸氢盐重吸收中起重要作用。

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