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首页> 外文期刊>American Journal of Physiology >Glycosaminoglycan polymerization may enable osmotically inactive Na+ storage in the skin.
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Glycosaminoglycan polymerization may enable osmotically inactive Na+ storage in the skin.

机译：糖胺聚糖聚合可以使渗透失活的Na +储存在皮肤中。

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摘要

Osmotically inactive skin Na(+) storage is characterized by Na(+) accumulation without water accumulation in the skin. Negatively charged glycosaminoglycans (GAGs) may be important in skin Na(+) storage. We investigated changes in skin GAG content and key enzymes of GAG chain polymerization during osmotically inactive skin Na(+) storage. Female Sprague-Dawley rats were fed a 0.1% or 8% NaCl diet for 8 wk. Skin GAG content was measured by Western blot analysis. mRNA content of key dermatan sulfate polymerization enzymes was measured by real-time PCR. The Na(+) concentration in skin was determined by dry ashing. Skin Na(+) concentration during osmotically inactive Na(+) storage was 180-190 mmol/l. Increasing skin Na(+) coincided with increasing GAG content in cartilage and skin. Dietary NaCl loading coincided with increased chondroitin synthase mRNA content in the skin, whereas xylosyl transferase, biglycan, and decorin content were unchanged. We conclude that osmotically inactive skin Na(+) storage is an active process characterized by an increased GAG content in the reservoir tissue. Inhibition or disinhibition of GAG chain polymerization may regulate osmotically inactive Na(+) storage.
机译：渗透性不活跃的皮肤Na（+）存储的特征在于Na（+）积累，皮肤中没有水积累。带负电荷的糖胺聚糖（GAGs）在皮肤Na（+）存储中可能很重要。我们调查了渗透活性的皮肤Na（+）存储过程中皮肤GAG含量和GAG链聚合关键酶的变化。给雌性Sprague-Dawley大鼠喂食0.1％或8％的NaCl饮食，持续8周。通过蛋白质印迹分析测量皮肤GAG含量。通过实时PCR测量关键的硫酸皮肤素聚合酶的mRNA含量。皮肤中的Na（+）浓度通过干灰测定。在无渗透性Na（+）存储过程中，皮肤Na（+）浓度为180-190 mmol / l。皮肤中Na（+）的增加与软骨和皮肤中GAG含量的增加相吻合。饮食中NaCl的摄入量与皮肤中软骨素合酶mRNA含量的增加同时发生，而木糖基转移酶，双糖链蛋白聚糖和核心蛋白聚糖的含量没有变化。我们得出的结论是，渗透性失活的皮肤Na（+）存储是一个活跃的过程，其特征是贮库组织中GAG含量增加。 GAG链聚合的抑制或去抑制可以调节渗透活性的Na（+）存储。

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