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首页> 外文期刊>American Journal of Physiology >Differential expression of collagen- and laminin-binding integrins mediates ureteric bud and inner medullary collecting duct cell tubulogenesis.
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Differential expression of collagen- and laminin-binding integrins mediates ureteric bud and inner medullary collecting duct cell tubulogenesis.

机译：胶原蛋白和层粘连蛋白结合整合素的差异表达介导输尿管芽和内髓收集导管细胞微管发生。

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摘要

Inner medullary collecting ducts (IMCD) are terminally differentiated structures derived from the ureteric bud (UB). UB development is mediated by changes in the temporal and spatial expression of integrins and their respective ligands. We demonstrate both in vivo and in vitro that the UB expresses predominantly laminin receptors (alpha3beta1-, alpha6beta1-, and alpha6beta(4-integrins), whereas the IMCD expresses both collagen (alpha1beta1- and alpha2beta1-integrins) and laminin receptors. Cells derived from the IMCD, but not the UB, undergo tubulogenesis in collagen-I (CI) gels in an alpha1beta1- and alpha2beta1-dependent manner. UB cells transfected with the alpha2-integrin subunit undergo tubulogenesis in CI, suggesting that collagen receptors are required for branching morphogenesis in CI. In contrast, both UB and IMCD cells undergo tubulogenesis in CI/Matrigel gels. UB cells primarily utilize alpha3beta1- and alpha6-integrins, whereas IMCD cells mainly employ alpha1beta1 for this process. These results demonstrate a switch in integrin expression from primarily laminin receptors in the early UB to both collagen and laminin receptors in the mature IMCD, which has functional consequences for branching morphogenesis in three-dimensional cell culture models. This suggests that temporal and spatial changes in integrin expression could help organize the pattern of branching morphogenesis of the developing collecting system in vivo.
机译：髓内收集管（IMCD）是源自输尿管芽（UB）的终末分化结构。 UB的发展是由整合素及其各自配体的时间和空间表达的变化介导的。我们在体内和体外均证实UB主要表达层粘连蛋白受体（alpha3beta1，alpha6beta1和alpha6beta（4-integrins），而IMCD同时表达胶原蛋白（alpha1beta1和alpha2beta1-integrins）和层粘连蛋白受体。来自IMCD而非UB的细胞通过胶原蛋白I（CI）凝胶以α1beta1和alpha2beta1依赖性的方式发生微管形成，转染有alpha2整合素亚基的UB细胞发生CI的微管形成，这表明胶原受体是必需的相比之下，UB和IMCD细胞在CI / Matrigel凝胶中均发生微管形成，UB细胞主要利用alpha3beta1和alpha6-integrins，而IMCD细胞主要利用alpha1beta1进行此过程，这些结果表明整合素表达发生了变化。从UB早期的主要层粘连蛋白受体到成熟的IMCD中的胶原蛋白和层粘连蛋白受体，对分支形态发生具有功能性影响在三维细胞培养模型中这表明整联蛋白表达的时空变化可以帮助组织体内发育中的采集系统的分支形态发生模式。

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