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首页> 外文期刊>American Journal of Physiology >Role of IL-10 in regulating proinflammatory cytokine release by Kupffer cells following trauma-hemorrhage.
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Role of IL-10 in regulating proinflammatory cytokine release by Kupffer cells following trauma-hemorrhage.

机译：IL-10在创伤性出血后调节库普弗细胞促炎细胞因子释放的作用。

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IL-6 and TNF-alpha production by Kupffer cells is markedly stimulated following trauma-hemorrhage (T-H). Because IL-10 is an anti-inflammatory cytokine, the aim of this study was to determine whether IL-10 regulates Kupffer cell proinflammatory cytokine release following T-H. To study this, we subjected adult male Sprague-Dawley rats to sham operation or T-H. The procedure involved a 5-cm midline laparotomy and approximately 90 min of hemorrhagic shock (35 mmHg), followed by resuscitation with four times the shed blood volume in the form of Ringer's lactate. At 2 h after the end of resuscitation, livers were perfused in vitro and perfusate was collected. In separate studies, Kupffer cells were isolated and incubated with different concentrations of anti-IL-10 MAb. IgG was used as control. After 16 h of incubation, IL-6 and TNF-alpha levels were measured by ELISA. Plasma IL-10 levels increased significantly following T-H. IL-10 levels in the perfusate and IL-10 production by cultured Kupffer cells were also significantly higher in the T-H group. When Kupffer cells were incubated with 10 microg/ml of anti-IL-10 MAb, IL-6 and TNF-alpha production were significantly increased in both sham and T-H groups compared with those not treated with anti-IL-10 MAb. However, these changes were not observed when the cells were incubated with irrelevant (control) IgG. These results indicate that IL-10 production by Kupffer cells early after T-H may play a pivotal role in attenuating the proinflammatory cytokine environment, possibly in an autocrine/paracrine manner.
机译：创伤性出血（T-H）后，库普弗细胞产生的IL-6和TNF-α受到明显刺激。因为IL-10是抗炎细胞因子，所以本研究的目的是确定IL-10是否在T-H后调节Kupffer细胞促炎细胞因子的释放。为了对此进行研究，我们对成年雄性Sprague-Dawley大鼠进行了假手术或T-H。该过程包括5 cm的中线剖腹手术和大约90分钟的失血性休克（35 mmHg），然后进行复苏，并以乳酸林格氏液的形式流血四倍。复苏结束后2小时，在体外灌注肝脏并收集灌注液。在单独的研究中，分离出库普弗细胞，并与不同浓度的抗IL-10 MAb孵育。 IgG用作对照。温育16小时后，通过ELISA测量IL-6和TNF-α水平。 T-H后血浆IL-10水平显着升高。在T-H组中，灌洗液中的IL-10水平和培养的Kupffer细胞产生的IL-10也明显较高。当库普弗细胞与10微克/毫升抗IL-10 MAb孵育时，与未接受抗IL-10 MAb处理的假手术组相比，假手术和T-H组的IL-6和TNF-α产量均显着增加。但是，将细胞与无关（对照）IgG孵育时未观察到这些变化。这些结果表明，T-H早期库普弗细胞产生IL-10可能以自分泌/旁分泌方式在减弱促炎细胞因子环境中起关键作用。

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