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首页> 外文期刊>American Journal of Physiology >L-Glutamine ameliorates acetaldehyde-induced increase in paracellular permeability in Caco-2 cell monolayer.
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L-Glutamine ameliorates acetaldehyde-induced increase in paracellular permeability in Caco-2 cell monolayer.

机译：L-谷氨酰胺改善了乙醛诱导的Caco-2细胞单层细胞旁通透性的增加。

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摘要

Role of L-glutamine in the protection of intestinal epithelium from acetaldehyde-induced disruption of barrier function was evaluated in Caco-2 cell monolayer. L-Glutamine reduced the acetaldehyde-induced decrease in transepithelilal electrical resistance and increase in permeability to inulin and lipopolysaccharide in a time- and dose-dependent manner; d-glutamine, L-aspargine, L-arginine, L-lysine, or L-alanine produced no significant protection. The glutaminase inhibitor 6-diazo-5-oxo-L-norleucine failed to affect the L-glutamine-mediated protection of barrier function. L-Glutamine reduced the acetaldehyde-induced redistribution of occludin, zonula occludens-1 (ZO-1), E-cadherin, and beta-catenin from the intercellular junctions. Acetaldehyde dissociates occludin, ZO-1, E-cadherin, and beta-catenin from the actin cytoskeleton, and this effect was reduced by L-glutamine. L-Glutamine induced a rapid increase in the tyrosine phosphorylation of EGF receptor, and the protective effect of L-glutamine was prevented by AG1478, the EGF-receptor tyrosine kinase inhibitor. These results indicate that L-glutamine prevents acetaldehyde-induced disruption of the tight junction and increase in the paracellular permeability in Caco-2 cell monolayer by an EGF receptor-dependent mechanism.
机译：在Caco-2细胞单层中评估了L-谷氨酰胺在保护肠上皮免受乙醛诱导的屏障功能破坏中的作用。 L-谷氨酰胺以时间和剂量依赖的方式减少了乙醛引起的跨上皮电阻的降低以及对菊粉和脂多糖的渗透性增加; d-谷氨酰胺，L-天冬氨酸，L-精氨酸，L-赖氨酸或L-丙氨酸未产生明显的保护作用。谷氨酰胺酶抑制剂6-重氮-5-氧-L-正亮氨酸不能影响L-谷氨酰胺介导的屏障功能保护。 L-谷氨酰胺减少了乙醛诱导的occludin，zonula occludens-1（ZO-1），E-cadherin和β-catenin从细胞间连接处的重新分布。乙醛使肌动蛋白细胞骨架中的occludin，ZO-1，E-cadherin和β-catenin解离，L-谷氨酰胺减弱了这种作用。 L-谷氨酰胺诱导EGF受体的酪氨酸磷酸化迅速增加，而EGF-受体酪氨酸激酶抑制剂AG1478阻止了L-谷氨酰胺的保护作用。这些结果表明，L-谷氨酰胺通过依赖EGF受体的机制阻止了乙醛诱导的紧密连接的破坏和Caco-2细胞单层细胞旁通透性的增加。

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