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首页> 外文期刊>American Journal of Physiology >Prolonged retention of the anorectic cobalt protoporphyrin in the hypothalamus and the resulting expression of Fos.
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Prolonged retention of the anorectic cobalt protoporphyrin in the hypothalamus and the resulting expression of Fos.

机译：下丘脑中厌食钴原卟啉的保留时间延长，并导致Fos表达。

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摘要

The anorectic cobalt protoporphyrin (CoPP) is known to elicit short-term hypophagia and long-term weight loss through unknown mechanisms in the brains of experimental animals. The goal of this work was to determine 1) if the prolonged duration of action of CoPP is related to its prolonged retention within the brain; and 2) with the use of immunohistochemical detection of Fos, the product of the early-immediate gene c-fos, which cells are activated after exposure to CoPP. These studies were carried out in male rats after intracerebroventricular administration of CoPP, 0.4 micromol/kg body wt, given under light halothane anesthesia. Residence of CoPP in the brain was determined by residual counts in dissected brains of (57)CoPP-injected rats. Fos immunoreactivity was mapped in coronal sections of rat brains 4-6 h after injection with CoPP. The results showed that (57)CoPP was retained in the hypothalamus preferentially compared with the cortex of the brain and could be detected in the hypothalamus for inexcess of 5 wk. Fos activation was increased by CoPP, detected predominantly in neuronal rather than glial cells, and was markedly more robust in the hypothalamus than in other brain areas. Thus CoPP remains in the hypothalamus for prolonged periods and activates Fos expression in the hypothalamus.
机译：已知厌食钴原卟啉（CoPP）通过实验动物大脑中的未知机制引起短期吞咽不足和长期体重减轻。这项工作的目的是确定1）CoPP作用的持续时间是否与其在大脑中的长期保留有关; 2）通过免疫组化检测Fos，即早期即时基因c-fos的产物，该细胞在暴露于CoPP后被激活。这些研究是在轻度氟烷麻醉下以0.4 micromol / kg体重的CoPP脑室内给药后在雄性大鼠中进行的。 CoPP在大脑中的驻留是由（57）个CoPP注射大鼠的解剖脑中的残留计数确定的。注射CoPP后4-6小时，将Fos免疫反应性定位在大鼠大脑的冠状切片中。结果表明，与大脑皮层相比，（57）CoPP优先保留在下丘脑中，并且在5 wk过剩时可以在下丘脑中检测到。 Fos活化通过CoPP增强，主要在神经元而不是神经胶质细胞中检测到，在下丘脑中比在其他大脑区域中明显更强。因此，CoPP长时间保留在下丘脑中并激活下丘脑中的Fos表达。

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