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首页> 外文期刊>American Journal of Physiology >Expression and localization of diacylglycerol kinase isozymes and enzymatic features in rat lung.
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Expression and localization of diacylglycerol kinase isozymes and enzymatic features in rat lung.

机译：二酰基甘油激酶同工酶在大鼠肺中的表达和定位及酶学特征。

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摘要

Diacylglycerol kinase (DGK) catalyzes phosphorylation of diacylglycerol to generate phosphatidic acid, and both molecules are known to serve as second messengers as well as important intermediates for the synthesis of various lipids. In this study, we investigated the spatiotemporal expression patterns of DGK isozymes together with the developmental changes of the mRNA expression and enzymatic property in rat lung. Northern blot and RT-PCR analyses showed that mRNAs for DGKalpha, -epsilon, and -zeta were detected in the lung. By immunohistochemical examination, DGKalpha and -zeta were shown to be coexpressed in alveolar type II cells and macrophages. Interestingly, these isozymes were localized at distinct subcellular locations, i.e., DGKalpha in the cytoplasm and DGKzeta in the nucleus, suggesting different roles for these isozymes. In the developing lung, the expression for DGKalpha and -zeta was transiently elevated on embryonic day 21 (E21) to levels approximately two- to threefold higher than on postnatal day 0 (P0). On the other hand, the expression for DGKepsilon was inversely elevated approximately twofold on P0 compared with that on E21. These unique changes in the expression pattern during the perinatal period suggest that each isozyme may play a distinct role in the adaptation of the lung to air or oxygen breathing at birth.
机译：二酰基甘油激酶（DGK）催化二酰基甘油的磷酸化以生成磷脂酸，并且已知这两个分子都可作为第二信使以及各种脂质合成的重要中间体。在这项研究中，我们调查了DGK同工酶的时空表达模式，以及大鼠肺中mRNA表达和酶学性质的发育变化。 Northern印迹和RT-PCR分析表明，在肺中检测到DGKalpha，-epsilon和-zeta的mRNA。通过免疫组织化学检查，显示DGKalpha和-zeta在肺泡II型细胞和巨噬细胞中共表达。有趣的是，这些同工酶位于不同的亚细胞位置，即细胞质中的DGKalpha和细胞核中的DGKzeta，提示这些同工酶的作用不同。在发育中的肺中，DGKalpha和-zeta的表达在胚胎第21天（E21）瞬时升高，比出生后第0天（P0）高约2至3倍。另一方面，与E21相比，P0上的DGKepsilon的表达反向升高约两倍。围生期表达模式的这些独特变化表明，每种同工酶可能在出生时肺对空气或氧气呼吸的适应中起着独特的作用。

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