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首页> 外文期刊>American Journal of Physiology >Cl- secretory effects of EBIO in the rabbit conjunctival epithelium.
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Cl- secretory effects of EBIO in the rabbit conjunctival epithelium.

机译：EBIO在兔结膜上皮中的Cl-分泌作用。

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摘要

Experiments were conducted to determine whether the Cl- secretagogue, 1-ethyl-2-benzimidazolinone (EBIO), stimulates Cl- transport in the rabbit conjunctival epithelium. For this study, epithelia were isolated in an Ussing-type chamber under short-circuit conditions. The effects of EBIO on the short-circuit current (I(sc)) and transepithelial resistance (R(t)) were measured under physiological conditions, as well as in experiments with altered electrolyte concentrations. Addition of 0.5 mM EBIO to the apical bath stimulated the control I(sc) by 64% and reduced R(t) by 21% (P < 0.05; paired data). Under Cl(-)-free conditions, I(sc) stimulation using EBIO was markedly attenuated. In the presence of an apical-to-basolateral K+ gradient and permeabilization of the apical membrane, the majority of the I(sc) reflected the transcellular movement of K+ via basolateral K+ channels. Under these conditions, EBIO in combination with A23187 elicited nearly instantaneous 60-90% increases in I(sc) that were sensitiveto the calmodulin antagonist calmidazolium and the K+ channel blocker tetraethyl ammonium. In the presence of an apical-to-basolateral Cl- gradient and nystatin permeabilization of the basolateral aspect, EBIO increased the Cl(-)-dependent I(sc), an effect prevented by the channel blocker glibenclamide (0.3 mM). The latter compound also was used to determine the proportion of EBIO-evoked unidirectional 36Cl- fluxes in the presence of the Cl- gradient that traversed the epithelium transcellularly. Overall, EBIO activated apical Cl- channels and basolateral K+ channels (presumably those that are Ca2+ dependent), thereby suggesting that this compound, or related derivatives, may be suitable as topical agents to stimulate fluid transport across the tissue in individuals with lacrimal gland deficiencies.
机译：进行实验以确定Cl-促分泌素1-乙基-2-苯并咪唑啉酮（EBIO）是否刺激兔结膜上皮中的Cl-转运。对于本研究，在短路条件下将上皮细胞隔离在Ussing型腔室中。在生理条件下以及在电解质浓度变化的实验中，都测量了EBIO对短路电流（I（sc））和跨上皮电阻（R（t））的影响。在顶浴中添加0.5 mM EBIO可使对照I（sc）升高64％，R（t）降低21％（P <0.05;配对数据）。在无Cl（-）的条件下，使用EBIO的I（sc）刺激明显减弱。在存在根尖到基底外侧的K +梯度和顶端膜的通透性时，大部分I（sc）反映了K +通过基底外侧K +通道的跨细胞运动。在这些条件下，EBIO与A23187结合会导致对钙调蛋白拮抗剂Calidazolium和K +通道阻滞剂四乙基铵敏感的I（sc）几乎立即增加60-90％。在根尖到基底外侧的Cl-梯度和制霉菌素透化的基底外侧方面，EBIO增加了Cl（-）依赖的I（sc），这是由通道阻滞剂格列本脲（0.3 mM）阻止的。在存在跨细胞穿过上皮的Cl-梯度的情况下，后一种化合物还用于确定EBIO诱发的单向36Cl-通量的比例。总体而言，EBIO激活了根尖的Cl-通道和基底外侧K +通道（可能是依赖Ca2 +的通道），因此表明该化合物或相关衍生物可能适合作为局部用药，刺激泪腺缺乏症患者的液体在组织中的运输。 。

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