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首页> 外文期刊>American Journal of Physiology >Functional alterations in cerebrovascular K(+) and Ca(2+) channels are comparable between simulated microgravity rat and SHR.
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Functional alterations in cerebrovascular K(+) and Ca(2+) channels are comparable between simulated microgravity rat and SHR.

机译：在模拟微重力大鼠和SHR之间的脑血管K（+）和Ca（2+）通道的功能改变是可比的。

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摘要

Exposure to microgravity leads to a sustained elevation in transmural pressure across the cerebral vasculature due to removal of hydrostatic pressure gradients. We hypothesized that ion channel remodeling in cerebral vascular smooth muscle cells (VSMCs) similar to that associated with hypertension may occur and play a role in upward autoregulation of cerebral vessels during microgravity. Sprague-Dawley rats were subjected to 4-wk tail suspension (Sus) to simulate the cardiovascular effect of microgravity. Large-conductance Ca(2+)-activated K(+) (BK(Ca)), voltage-gated K(+) (K(V)), and L-type voltage-dependent Ca(2+) (Ca(L)) currents of Sus and control (Con) rat cerebral VSMCs were investigated with a whole cell voltage-clamp technique. Under the same experimental conditions, K(V), BK(Ca), and Ca(L) currents of cerebral VSMCs from adult spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY) were also investigated. K(V) current density decreased in Sus rats vs. Con rats [1.07 +/- 0.14 (n = 22)vs. 1.31 +/- 0.28 (n = 16) pA/pF at +20 mV (P < 0.05)] and BK(Ca) and Ca(L) current densities increased [BK(Ca): 1.70 +/- 0.37 (n = 23) vs. 0.88 +/- 0.22 (n = 19) pA/pF at +20 mV (P < 0.05); Ca(L): -2.17 +/- 0.21 (n = 35) vs. -1.31 +/- 0.10 (n = 26) pA/pF at +10 mV (P < 0.05)]. Similar changes were also observed in SHR vs. WKY cerebral VSMCs: K(V) current density decreased [1.03 +/- 0.33 (n = 9) vs. 1.62 +/- 0.64 (n = 9) pA/pF at +20 mV (P < 0.05)] and BK(Ca) and Ca(L) current densities increased [BK(Ca): 2.54 +/- 0.47 (n = 11) vs. 1.12 +/- 0.33 (n = 12) pA/pF at +20 mV (P < 0.05); Ca(L): -3.99 +/- 0.53 (n = 12) vs. -2.28 +/- 0.20 (n = 10) pA/pF at +20 mV (P < 0.05)]. These findings support our hypothesis, and their impact on space cardiovascular research is discussed.
机译：由于去除了静水压力梯度，暴露于微重力下导致跨脑血管的跨壁压力持续升高。我们假设可能发生类似于与高血压相关的脑血管平滑肌细胞（VSMC）的离子通道重塑，并在微重力作用下对脑血管的向上自动调节起作用。对Sprague-Dawley大鼠进行4-wk尾部悬吊（Sus），以模拟微重力的心血管作用。大电导Ca（2+）激活的K（+）（BK（Ca）），电压门控K（+）（K（V））和L型电压依赖性Ca（2+）（Ca（ L））用全细胞电压钳技术研究了Sus和对照（Con）大鼠脑VSMC的电流。在相同的实验条件下，还研究了成年自发性高血压大鼠（SHR）和Wistar-Kyoto大鼠（WKY）的脑VSMC的K（V），BK（Ca）和Ca（L）电流。 Sus大鼠和Con大鼠的K（V）电流密度降低[1.07 +/- 0.14（n = 22）vs。 +20 mV时1.31 +/- 0.28（n = 16）pA / pF（P <0.05）]，BK（Ca）和Ca（L）电流密度增加[BK（Ca）：1.70 +/- 0.37（n = 23）与+20 mV时的0.88 +/- 0.22（n = 19）pA / pF（P <0.05）; Ca（L）：+ 10 mV时-2.17 +/- 0.21（n = 35）与-1.31 +/- 0.10（n = 26）pA / pF（P <0.05）]。在SHR与WKY脑VSMC中也观察到了类似的变化：+20 mV时，K（V）电流密度降低了[1.03 +/- 0.33（n = 9）vs. 1.62 +/- 0.64（n = 9）pA / pF （P <0.05）]以及BK（Ca）和Ca（L）电流密度增加[BK（Ca）：2.54 +/- 0.47（n = 11）与1.12 +/- 0.33（n = 12）pA / pF在+20 mV时（P <0.05）; Ca（L）：在+20 mV时-3.99 +/- 0.53（n = 12）与-2.28 +/- 0.20（n = 10）pA / pF（P <0.05）]。这些发现支持我们的假设，并讨论了它们对空间心血管研究的影响。

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