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首页> 外文期刊>American Journal of Physiology >A novel type of urea transporter, UT-C, is highly expressed in proximal tubule of seawater eel kidney.
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A novel type of urea transporter, UT-C, is highly expressed in proximal tubule of seawater eel kidney.

机译：新型的尿素转运蛋白UT-C在海水鳗肾近端小管中高度表达。

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摘要

A new type of urea transporter was identified by a database search and shown to be highly expressed in the renal proximal tubule cells of teleosts; proximal tubule-type urea transporters have not been describe previously. We first identified urea transporter-like sequences in the fugu genome and in an EST database of rainbow trout. Based on these pieces of sequence information, we obtained a full-length cDNA for the eel ortholog, consisting of 378 amino acid residues, and named it eUT-C. Although its sequence similarity to the known urea transporters is low (approximately 35%), its heterologous expression in Xenopus laevis oocytes indicated that it is a facilitative urea transporter sensitive to phloretin. Its activity is not dependent on Na+. Northern blot analysis showed that expression of eUT-C is highly restricted to the kidney, with weak expression in the stomach. In both tissues, eUT-C mRNA was strongly induced when eels were transferred from freshwater to seawater. Immunohistochemistry and in situ hybridization histochemistry revealed proximal tubule cell localization of eUT-C. Taking into account that 1) urea is mainly secreted from the gill where another type of urea transporter (eUT) has been identified and 2) fish excrete a very small volume of urine in seawater, we propose that eUT-C cloned here is a key component working in combination with the gill transporter to achieve an efficient urea excretory system in fish, namely, eUT-C reabsorbs urea from glomerular filtrate and sends it to the gill, through the circulation, for excretion.
机译：通过数据库搜索确定了一种新型的尿素转运蛋白，并显示其在硬骨鱼的肾近端小管细胞中高表达。近端肾小管型尿素转运蛋白以前没有描述。我们首先在河豚基因组和虹鳟的EST数据库中确定了类似尿素转运蛋白的序列。根据这些序列信息，我们获得了鳗鱼直系同源基因的全长cDNA，由378个氨基酸残基组成，并将其命名为eUT-C。尽管其与已知尿素转运蛋白的序列相似性较低（约35％），但其在非洲爪蟾卵母细胞中的异源表达表明它是对毛孔菌素敏感的促进尿素转运蛋白。其活性不依赖于Na +。 Northern印迹分析表明，eUT-C的表达高度限于肾脏，在胃中表达较弱。在鳗鱼从淡水转移到海水中时，在两种组织中都强烈诱导了eUT-C mRNA的表达。免疫组织化学和原位杂交组织化学揭示了eUT-C的近端小管细胞定位。考虑到1）尿素主要是从the中分泌出来的，在那里已经发现了另一种类型的尿素转运蛋白（eUT），并且2）鱼在海水中排泄了很少量的尿液，因此我们建议此处克隆的eUT-C是关键该组件与the运输器一起工作，以实现鱼类中有效的尿素排泄系统，即eUT-C从肾小球滤液中重新吸收尿素，并通过循环将其发送至g进行排泄。

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