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首页> 外文期刊>American Journal of Physiology >Regulation of maturation and processing of ENaC subunits in the rat kidney.
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Regulation of maturation and processing of ENaC subunits in the rat kidney.

机译：调节大鼠肾脏中ENaC亚基的成熟和加工。

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摘要

Antibodies directed against subunits of the epithelial Na channel (ENaC) were used together with electrophysiological measurements in the cortical collecting duct to investigate the processing of the proteins in rat kidney with changes in Na or K intake. When animals were maintained on a low-Na diet for 7-9 days, the abundance of two forms of the alpha-subunit, with apparent masses of 85 and 30 kDa, increased. Salt restriction also increased the abundance of the beta-subunit and produced an endoglycosidase H (Endo H)-resistant pool of this subunit. The abundance of the 90-kDa form of the gamma-subunit decreased, whereas that of a 70-kDa form increased and this peptide also exhibited Endo H-resistant glycosylation. These changes in alpha- and gamma-subunits were correlated with increases in Na conductance elicited by a 4-h infusion with aldosterone. Changes in all three subunits were correlated with decreases in Na conductance when Na-deprived animals drank saline for 5 h. We conclude that ENaC subunitsare mainly in an immature form in salt-replete rats. With Na depletion, the subunits mature in a process that involves proteolytic cleavage and further glycosylation. Similar changes occurred in alpha- and gamma- but not beta-subunits when animals were treated with exogenous aldosterone, and in beta- and gamma- but not alpha-subunits when animals were fed a high-K diet. Changes in the processing and maturation of the channels occur rapidly enough to be involved in the daily regulation of ENaC activity and Na reabsorption by the kidney.
机译：针对皮质上皮Na通道（ENaC）亚基的抗体与皮层收集管中的电生理测量一起使用，以研究随着Na或K摄入量变化而在大鼠肾脏中加工蛋白质的过程。当动物以低钠饮食维持7-9天时，两种形式的α-亚基（表观质量分别为85 kDa和30 kDa）的丰度增加了。盐限制也增加了β-亚基的丰度，并产生了该亚基的内切糖苷酶H（Endo H）抗性库。 γ亚基的90 kDa形式的丰度降低，而70 kDa形式的丰度增加，并且该肽还表现出Endo H抗性糖基化。这些α-和γ-亚基的变化与醛固酮4小时输注引起的Na电导增加有关。当缺钠的动物喝盐水5 h时，所有三个亚基的变化都与钠电导的降低相关。我们得出结论，在食盐丰富的大鼠中，ENaC亚基主要以未成熟形式存在。随着钠的消耗，亚基在涉及蛋白水解切割和进一步糖基化的过程中成熟。当用外源性醛固酮治疗动物时，α-和γ-亚基发生了类似的变化，而高钾饮食喂养的动物，β-和γ-亚基发生了类似的变化。通道加工和成熟的变化发生得足够快，足以参与ENaC活性和肾脏对Na重吸收的日常调节。

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