• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >A novel bronchial ring bioassay for the evaluation of small airway smooth muscle function in mice.
【24h】

A novel bronchial ring bioassay for the evaluation of small airway smooth muscle function in mice.

机译：一种新颖的支气管环生物测定法，用于评估小鼠小气道平滑肌功能。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Advances in our understanding of murine airway physiology have been hindered by the lack of suitable, ex vivo, small airway bioassay systems. In this study, we introduce a novel small murine airway bioassay system that permits the physiological and pharmacological study of intrapulmonary bronchial smooth muscle via a bronchial ring (BR) preparation utilizing BR segments as small as 200 microm in diameter. Using this ex vivo BR bioassay, we characterized small airway smooth muscle contraction and relaxation in the presence and absence of bronchial epithelium. In control BRs, the application of mechanical stretch is followed by spontaneous bronchial smooth muscle relaxation. BRs pretreated with methacholine (MCh) partially attenuate this stretch-induced relaxation by as much as 42% compared with control. MCh elicited a dose-dependent bronchial constriction with a maximal tension (E(max)) of 8.7 +/- 0.2 mN at an EC(50) of 0.33 +/- 0.02 microM. In the presence of nifedipine, ryanodine, 2-aminoethoxydiphenyl borate, and SKF-96365, E(max) to MCh was significantly reduced. In epithelium-denuded BRs, MCh-induced contraction was significantly enhanced to 11.4 +/- 1.0 mN with an EC(50) of 0.16 +/- 0.04 microM (P < 0.01). Substance P relaxed MCh-precontracted BR by 62.1%; however, this bronchial relaxation effect was completely lost in epithelium-denuded BRs. Papaverine virtually abolished MCh-induced constriction in both epithelium-intact and epithelium-denuded bronchial smooth muscle. In conclusion, this study introduces a novel murine small airway BR bioassay that allows for the physiological study of smooth muscle airway contractile responses that may aid in our understanding of the pathophysiology of asthma.
机译：由于缺乏合适的离体小型气道生物测定系统，阻碍了我们对鼠气道生理学的理解。在这项研究中，我们介绍了一种新型的小型鼠类气道生物测定系统，该系统可通过支气管环（BR）制备物利用直径小于200微米的BR段，对肺内支气管平滑肌进行生理和药理研究。使用这种离体BR生物测定法，我们在存在和不存在支气管上皮的情况下表征了小气道平滑肌的收缩和松弛。在对照BR中，机械拉伸的应用是自发性支气管平滑肌松弛。与对照相比，用乙酰甲胆碱（MCh）预处理的BR可以部分地将这种拉伸诱导的松弛作用减弱42％。 MCh引发剂量依赖性的支气管收缩，在EC（50）为0.33 +/- 0.02 microM时，最大张力（E（max））为8.7 +/- 0.2 mN。在硝苯地平，ryanodine，2-氨基乙氧基二苯基硼酸盐和SKF-96365的存在下，MCh的E（max）显着降低。在上皮裸露的BRs中，MCh诱导的收缩显着增强至11.4 +/- 1.0 mN，EC（50）为0.16 +/- 0.04 microM（P <0.01）。 P物质使MCh预缔合的BR减少了62.1％;但是，这种支气管舒张作用在上皮裸露的BR中完全消失了。罂粟碱实际上消除了MCh诱导的上皮完整和上皮裸露的支气管平滑肌的收缩。总之，本研究引入了一种新型的鼠小气道BR生物测定法，可用于平滑肌气道收缩反应的生理学研究，这可能有助于我们了解哮喘的病理生理学。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号