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首页> 外文期刊>American Journal of Physiology >Lung surfactant secretion by interalveolar Ca2+ signaling.
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Lung surfactant secretion by interalveolar Ca2+ signaling.

机译：肺泡表面活性剂通过肺泡内Ca2 +信号传导分泌。

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摘要

Although clusters of alveoli form the acinus, which is the most distal respiratory unit, it is not known whether interalveolar communication coordinates acinar surfactant secretion. To address this, we applied real-time digital imaging in conjunction with photo-excited Ca2+ uncaging in intact alveoli of the isolated, blood-perfused rat lung. We loaded alveolar cells with the Ca2+ cage o-nitrophenyl EGTA-AM (NP-EGTA-AM) together with the fluorophores, fluo 4, or LysoTracker green (LTG) to determine, respectively, the cytosolic Ca2+ concentration ([Ca2+]cyt) or type 2 cell secretion. To uncage Ca2+ from NP-EGTA, we exposed a region in a selected alveolus to high-intensity UV illumination. As a result, fluo 4 fluorescence increased, whereas LTG fluorescence decreased, in the photo-targeted region, indicating that uncaging both increased [Ca2+]cyt and induced secretion. Concomitantly, [Ca2+]cyt increases conducted from the uncaging site induced type 2 cell secretion in both the selected alveolus as well asin neighboring alveoli, indicating the presence of interalveolar communication. These conducted responses were inhibited by pretreating alveoli with the connexin43 (Cx43)-inhibiting peptides gap 26 and gap 27. However, although the conducted [Ca2+]cyt increase diminished with distance from the uncaging site, type 2 cell secretion rates were similar at all locations. We conclude that Cx43-dependent, interalveolar Ca2+ signals regulate type 2 cell secretion in adjacent alveoli. Such interalveolar communication might facilitate acinar coordination of alveolar function.
机译：尽管肺泡簇形成了最远端呼吸单元的腺泡，但目前尚不知道肺泡间的通讯是否协调腺泡表面活性剂的分泌。为了解决这个问题，我们将实时数字成像与光激发的Ca2 +解封结合在离体的血液灌注大鼠肺的完整肺泡中。我们用Ca2 +笼邻硝基苯基EGTA-AM（NP-EGTA-AM）以及荧光团，fluo 4或LysoTracker green（LTG）加载肺泡细胞，分别测定胞浆中Ca2 +的浓度（[Ca2 +] cyt）或2型细胞分泌物。为了从NP-EGTA解开Ca2 +，我们将选定的肺泡中的一个区域暴露于高强度紫外线照射下。结果，在受光靶向的区域中，fluo 4荧光增加，而LTG荧光减少，这表明解开时[Ca2 +] cyt和诱导的分泌均增加。伴随地，在选定的肺泡以及邻近的肺泡中，由解笼位点诱导的2型细胞分泌引起的[Ca2 +] cyt升高，表明存在肺泡间通讯。通过用连接蛋白43（Cx43）抑制肽间隙26和间隙27对肺泡进行预处理，可以抑制这些传导反应。但是，尽管[Ca2 +] cyt传导的增加随着与解笼位点距离的增加而减小，但是2型细胞的分泌速率在所有情况下都相似位置。我们得出的结论是Cx43依赖的，肺泡间Ca2 +信号调节相邻肺泡中的2型细胞分泌。这种牙槽间的交流可能有助于腺泡协调肺泡功能。

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