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首页> 外文期刊>American Journal of Physiology >Cloning of rainbow trout SLC26A1: involvement in renal sulfate secretion.
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Cloning of rainbow trout SLC26A1: involvement in renal sulfate secretion.

机译：虹鳟鱼SLC26A1的克隆：参与肾脏硫酸盐的分泌。

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摘要

The kidney plays an important role in ion regulation in both freshwater and seawater fish. However, ion transport mechanisms in the teleost kidney are poorly understood, especially at the molecular level. We have cloned a kidney-specific SLC26 sulfate/anion exchanger from rainbow trout (Oncorhynchus mykiss) that is homologous to the mammalian SLC26A1 (Sat-1). Excretion of excess plasma sulfate concentration after Na2SO4 injection corresponded to significantly higher expression of the cloned SLC26A1 mRNA. Detailed morphological observation of rainbow trout renal tubules was also performed by light microscopy and transmission electron microscopy. According to the structure of brush border and tubular system in the cytoplasm, renal tubules of rainbow trout were classified into proximal tubule first and second (PI and PII) segments and distal tubules. In situ hybridization revealed that SLC26A1 anion exchanger mRNA is specifically localized in the PI segment of kidneys from both seawater- and freshwater-adapted rainbow trout. With immunocytochemistry, Na+-K+-ATPase and vacuolar-type H+-ATPase were colocalized to the same cells and distributed in the basolateral and the apical membranes, respectively, of the cells where the SLC26A1 mRNA expressed. These findings suggest that the cloned kidney-specific SLC26A1 is located in kidney proximal tubules and is involved in excretion of excess plasma sulfate in rainbow trout.
机译：肾脏在淡水和海水鱼的离子调节中起着重要作用。但是，硬骨肾中的离子转运机制了解甚少，尤其是在分子水平上。我们已经从与鳟鱼SLC26A1（Sat-1）同源的虹鳟鱼（Oncorhynchus mykiss）中克隆了一种肾脏特异性SLC26硫酸盐/阴离子交换剂。注射Na2SO4后多余的血浆硫酸盐浓度的排泄对应于克隆的SLC26A1 mRNA的显着较高表达。虹鳟肾小管的详细形态学观察也通过光学显微镜和透射电子显微镜进行。根据细胞质中刷状缘和肾小管系统的结构，虹鳟的肾小管分为近端小管的第一和第二段（PI和PII）和远端小管。原位杂交表明，SLC26A1阴离子交换子mRNA特异地位于来自海水和淡水的虹鳟鱼肾脏的PI段中。通过免疫细胞化学，将Na + -K + -ATPase和液泡型H + -ATPase共定位在同一细胞中，并分别分布在表达SLC26A1 mRNA的细胞的基底外侧和顶膜中。这些发现表明，克隆的肾脏特异性SLC26A1位于肾脏近端小管中，并参与虹鳟鱼中过量血浆硫酸盐的排泄。

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