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首页> 外文期刊>American Journal of Physiology >UT-A urea transporter promoter, UT-Aalpha, targets principal cells of the renal inner medullary collecting duct.
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UT-A urea transporter promoter, UT-Aalpha, targets principal cells of the renal inner medullary collecting duct.

机译：UT-A尿素转运蛋白启动子UT-Aalpha靶向肾脏内髓收集管的主要细胞。

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摘要

The urea transporters, UT-A1 and UT-A3, two members of the UT-A gene family, are localized to the terminal portion of the inner medullary collecting duct (IMCD). In this manuscript, we demonstrate that 4.2 kb of the 5'-flanking region of the UT-A gene (UT-Aalpha promoter) is sufficient to drive the IMCD-specific expression of a heterologous reporter gene, beta-galactosidase (beta-Gal), in transgenic mice. RT-PCR, immunoblotting, and immunohistochemistry demonstrate that within the kidney, transgene expression is confined to the terminal portion of the IMCD. Colocalization studies with aquaporin-2 show that expression is localized to the principal cells of the IMCD2 and IMCD3 regions. Utilizing beta-Gal activity assays, we further show that within the kidney, the beta-Gal transgene can be regulated by both water restriction and glucocorticoids, similar to the regulation of the endogenous UT-A gene. These results demonstrate that 4.2 kb of the UT-Aalpha promoter is sufficient to drive expression of a heterologous reporter gene in a tissue-specific and cell-specific fashion in transgenic mice.
机译：尿素转运蛋白UT-A1和UT-A3是UT-A基因家族的两个成员，位于内髓收集管（IMCD）的末端。在此手稿中，我们证明了UT-A基因（UT-Aalpha启动子）5'侧翼区域的4.2 kb足以驱动异源报道基因β-半乳糖苷酶（β-Gal）的IMCD特异性表达），在转基因小鼠中。 RT-PCR，免疫印迹和免疫组织化学证明，在肾脏中，转基因表达仅限于IMCD的末端。与aquaporin-2的共定位研究表明，表达局限于IMCD2和IMCD3区的主要细胞。利用β-Gal活性测定法，我们进一步显示，在肾脏内，β-Gal转基因可以通过水限制和糖皮质激素来调节，类似于内源性UT-A基因的调节。这些结果表明，在转基因小鼠中，4.2 kb的UT-Aalpha启动子足以驱动异源报告基因以组织特异性和细胞特异性方式表达。

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