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首页> 外文期刊>American Journal of Physiology >Regulation of ecto-5'-nucleotidase by NaCl and nitric oxide: potential roles in tubuloglomerular feedback and adaptation.
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Regulation of ecto-5'-nucleotidase by NaCl and nitric oxide: potential roles in tubuloglomerular feedback and adaptation.

机译：NaCl和一氧化氮对ecto-5'核苷酸酶的调节：在肾小管肾小球反馈和适应中的潜在作用。

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The tubuloglomerular feedback (TGF) system serves to establish an appropriate balance between tubular reabsorption and glomerular filtration rate (GFR). High salt at the macula densa activates TGF to decrease GFR. Effector molecules for the TGF signal include ATP and adenosine. Over time, the GFR will adapt by increasing even if a high salt concentration persists. A potential modulator of this TGF adaptation is nitric oxide synthase-1-derived nitric oxide (NO). In isolated glomerular preparations, we developed a system for evaluating the effects of changing dietary salt on ecto-5'-nucleotidase (ecto-5'-NT) activity, the final enzyme in the conversion of ATP to adenosine. We found observable ecto-5'-NT activity in isolated glomeruli and that this activity can be regulated by dietary salt, with high salt increasing activity. Conversely, NO decreases ecto-5'-NT activity in glomerular preparations. Moreover, NO inhibition of ecto-5'-NT activity is suppressed in the presence of dithiothreitol, suggesting nitrosylation as a reversible, oxidative stress-sensitive mechanism. The salt-induced activation of ecto-5'-NT correlates with high salt resetting of TGF. NO inhibition of enzymatic activity could be part of the adaptive phase.
机译：肾小管肾小球反馈（TGF）系统用于在肾小管重吸收和肾小球滤过率（GFR）之间建立适当的平衡。黄斑部的高盐会激活TGF以降低GFR。用于TGF信号的效应分子包括ATP和腺苷。随着时间的推移，即使高盐浓度持续存在，GFR也会通过增加来适应。这种TGF适应的潜在调节剂是一氧化氮合酶1衍生的一氧化氮（NO）。在分离的肾小球制剂中，我们开发了一种系统，用于评估饮食盐对ecto-5'-核苷酸酶（ecto-5'-NT）活性（ATP转换为腺苷的最终酶）的影响。我们发现在分离的肾小球中可观察到的ecto-5'-NT活性，并且该活性可以通过饮食中的盐来调节，并具有较高的盐增加活性。相反，NO降低肾小球制剂中ecto-5'-NT的活性。此外，在二硫苏糖醇的存在下，NO对ecto-5'-NT活性的抑制被抑制，这表明亚硝基化是一种可逆的，氧化应激敏感的机制。盐诱导的ecto-5'-NT活化与TGF的高盐重置有关。没有抑制酶活性可能是适应期的一部分。

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