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首页> 外文期刊>American Journal of Physiology >Myometrial cells induce angiogenesis and salvage damaged myocardium.
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Myometrial cells induce angiogenesis and salvage damaged myocardium.

机译：肌层细胞诱导血管生成并挽救受损的心肌。

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Characteristically, uterine myometrial cells (MCs) are proliferative, inducing angiogenesis within the female reproductive organ. We evaluated whether MCs implanted into myocardium could also induce angiogenesis and restore heart function after injury. MCs were isolated from the adult rat uterus and cultured for three studies: 1) Intracellular VEGF levels were measured in MCs cultured with progesterone (10(-11), 10(-9), and 10(-7) M) (n = 6 tests per group). 2) Blood vessel density was evaluated 8 days after MCs (3 x 10(6) or 6 x 10(6)), smooth muscle cells (SMCs), or endothelial cells (n = 6 rats per group) were injected with matrigel into the subcutaneous tissue of adult rats. 3) MCs, SMCs (5 x 10(6)/rat), or media were injected into a transmural scar 3 wk after cryoinjury in rat hearts (n = 12 rats per group), and heart function, blood vessel density, and myocardial scar size and thickness were evaluated 5 wk later. In study 1, cultured MCs expressed VEGF, with levels significantly (P < 0.05) upregulated by progesterone at an optimal dose of 10(-11) M. In study 2, MCs injected into the subcutaneous tissue with matrigel induced significantly more blood vessels, especially large-diameter vessels, than did SMCs or endothelial cells (P < 0.01 for all groups). This angiogenic effect was greatest (P < 0.01) at higher doses of MCs and was enhanced by progesterone (10(-11) M). In study 3, MCs implanted into the injured myocardium increased blood vessel density at the implant area, reduced scar size, and improved cardiac function relative to SMCs and media. Overall, MCs induced angiogenesis in vitro and in vivo, prevented cardiac remodeling, and improved heart functional recovery after cardiac injury.
机译：子宫肌层细胞（MCs）具有增生作用，可诱导女性生殖器官内的血管生成。我们评估了植入心肌的MCs是否还能在损伤后诱导血管生成并恢复心脏功能。从成年大鼠子宫中分离出MC，并进行三项研究培养：1）在孕酮（10（-11），10（-9）和10（-7）M）培养的MC中测量细胞内VEGF水平（n =每组6个测试）。 2）在将MC（3 x 10（6）或6 x 10（6）），平滑肌细胞（SMC）或内皮细胞（每组6只大鼠）注射基质胶后8天，评估血管密度成年大鼠的皮下组织。 3）在大鼠心脏冷冻损伤后3周（每组12只大鼠），将MC，SMC（5 x 10（6）/只大鼠）或培养基注射到透壁瘢痕中（每组12只大鼠），以及心脏功能，血管密度和心肌5周后评估疤痕的大小和厚度。在研究1中，培养的MCs表达VEGF，在最佳剂量10（-11）M时，孕激素显着上调了VEGF水平（P <0.05）。在研究2中，基质胶注射入皮下组织的MCs诱导了更多的血管，尤其是大直径的血管，而不是SMC或内皮细胞（所有组的P <0.01）。在较高剂量的MCs中，这种血管生成作用最大（P <0.01），而孕酮（10（-11）M）增强了血管生成作用。在研究3中，相对于SMC和培养基，将MC植入受伤的心肌中可增加植入区域的血管密度，减少疤痕大小并改善心脏功能。总体而言，MC在体外和体内诱导血管生成，防止心脏重构，并改善心脏损伤后的心脏功能恢复。

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