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首页> 外文期刊>American Journal of Physiology >Measurement of myocardial free radical production during exercise using EPR spectroscopy.
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Measurement of myocardial free radical production during exercise using EPR spectroscopy.

机译：使用EPR光谱仪测量运动过程中心肌自由基的产生。

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摘要

Exercise is associated with an increase in oxygen flux through the mitochondrial electron transport chain that has recently been demonstrated to increase the production of reactive oxygen species (ROS) in skeletal muscle. This study examined whether exercise also causes free radical production in the heart. We measured ROS production in seven chronically instrumented dogs during rest and treadmill exercise (6.4 km/h at 10 degrees grade; and heart rate, 204 +/- 3 beats/min) using electron paramagnetic resonance spectroscopy in conjunction with the spin trap alpha-phenyl-tert-butylnitrone (PBN) (0.14 mol/l) in blood collected from the aorta and coronary sinus (CS). To improve signal detection, the free radical adducts were deoxygenated over a nitrogen stream for 15 min and extracted with toluene. The hyperfine splitting constants of the radicals were alpha(N) = 13.7 G and alpha(H) = 1.0 G, consistent with an alkoxyl or carbon-centered radical. Resting aortic and CS PBN adduct concentrations were 6.7 and 6.3 x 10(8) arbitrary units (P = not significant). Both aortic and CS adduct concentrations increased during exercise, but there was no significant difference between the aortic and CS concentrations. Thus, in contrast to skeletal muscle, submaximal treadmill exercise did not result in detectable free radical production by the heart.
机译：运动与通过线粒体电子传输链的氧通量的增加有关，最近已证明该运动可增加骨骼肌中活性氧物质（ROS）的产生。这项研究检查了运动是否还会引起心脏自由基的产生。我们使用电子顺磁共振波谱结合自旋阱α-测量了七只长期使用仪器的狗在休息和跑步机运动（10度坡度时每小时6.4 km / h，心率204 +/- 3拍/分钟）期间产生的ROS。从主动脉和冠状窦（CS）收集的血液中的苯基叔丁基硝基（PBN）（0.14 mol / l）。为了改善信号检测，将自由基加合物在氮气流中脱氧15分钟，然后用甲苯萃取。自由基的超细分裂常数为alpha（N）= 13.7 G和alpha（H）= 1.0 G，与烷氧基或碳中心自由基一致。静息主动脉和CS PBN加合物的浓度分别为6.7和6.3 x 10（8）任意单位（P =不显着）。运动期间主动脉和CS的加合物浓度均增加，但主动脉和CS的浓度之间无显着差异。因此，与骨骼肌相反，次最大的跑步机运动不会导致心脏产生可检测的自由基。

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