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首页> 外文期刊>American Journal of Physiology >Exercise training induces respiratory substrate-specific decrease in Ca2+-induced permeability transition pore opening in heart mitochondria.
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Exercise training induces respiratory substrate-specific decrease in Ca2+-induced permeability transition pore opening in heart mitochondria.

机译：运动训练在心脏线粒体中诱导Ca2 +诱导的通透性转换孔开放中呼吸底物特异性降低。

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摘要

The purpose of this study was to determine whether regular exercise (treadmill running, 10 wk) alters the susceptibility of rat isolated heart mitochondria to Ca(2+)-induced permeability transition pore (PTP) opening and whether this could be associated with changes in the modulation of PTP opening by selected physiological effectors. Basal leak-driven and ADP-stimulated respiration in the presence of substrates for complex I, II, and IV were not affected by training. Fluorimetric studies revealed that in the control and exercise-trained groups, the amount of Ca(2+) required to trigger PTP opening was greater in the presence of complex II vs. I substrates (230 +/- 12 vs. 134 +/- 7 nmol Ca(2+)/mg protein, P < 0.01; pooled average of control and trained groups). In addition, with a substrate feeding the complex II, training increased by 45% (P < 0.01) the amount of Ca(2+) required to trigger PTP opening both in the presence and absence of the PTP inhibitor cyclosporin A. However, membrane potential, reactive oxygen species production, NAD(P)H ratio, and Ca(2+) uptake kinetics were not different in mitochondria from both groups. Together, these results suggest the existence of a substrate-specific regulation of the PTP in heart mitochondria and suggest that regular exercise results in a reduced sensitivity to Ca(2+)-induced PTP opening in presence of complex II substrates.
机译：这项研究的目的是确定常规运动（跑步机，10 wk跑步）是否会改变大鼠离体心脏线粒体对Ca（2+）诱导的通透性转化孔（PTP）开口的敏感性，以及是否可能与改变通过选定的生理效应因子调节PTP的开放。在存在复杂I，II和IV底物的情况下，基础泄漏驱动和ADP刺激的呼吸不受训练的影响。荧光分析显示，在对照组和运动训练组中，在存在复杂的II对I底物的情况下，触发PTP开放所需的Ca（2+）量更大（230 +/- 12与134 +/- 7 nmol Ca（2 +）/ mg蛋白，P <0.01;对照组和训练组的合并平均值）。此外，在基质向复合物II供料的情况下，在存在和不存在PTP抑制剂环孢菌素A的情况下，训练都会使触发PTP打开所需的Ca（2+）量增加45％（P <0.01）。电位，活性氧的产生，NAD（P）H比和Ca（2+）吸收动力学在两组的线粒体中没有差异。在一起，这些结果表明在心脏线粒体中存在PTP的底物特异性调节，并表明在复杂的II底物的存在下，经常运动会导致对Ca（2+）诱导的PTP开放的敏感性降低。

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