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首页> 外文期刊>American Journal of Physiology >Age-associated decrease in muscle precursor cell differentiation.
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Age-associated decrease in muscle precursor cell differentiation.

机译：与年龄相关的肌肉前体细胞分化减少。

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Muscle precursor cells (MPCs) are required for the regrowth, regeneration, and/or hypertrophy of skeletal muscle, which are deficient in sarcopenia. In the present investigation, we have addressed the issue of age-associated changes in MPC differentiation. MPCs, including satellite cells, were isolated from both young and old rat skeletal muscle with a high degree of myogenic purity (>90% MyoD and desmin positive). MPCs isolated from skeletal muscle of 32-mo-old rats exhibited decreased differentiation into myotubes and demonstrated decreased myosin heavy chain (MHC) and muscle creatine kinase (CK-M) expression compared with MPCs isolated from 3-mo-old rats. p27(Kip1) is a cyclin-dependent kinase inhibitor that has been shown to enhance muscle differentiation in culture. Herein we describe our finding that p27(Kip1) protein was lower in differentiating MPCs from skeletal muscle of 32-mo-old rats than in 3-mo-old rat skeletal muscle. Although MHC and CK-M expression were approximately 50% lower in differentiating MPCs isolated from 32-mo-old rats, MyoD protein content was not different and myogenin protein concentration was twofold higher. These data suggest that there are inherent differences in cell signaling during the transition from cell cycle arrest to the formation of myotubes in MPCs isolated from sarcopenic muscle. Furthermore, there is an age-associated decrease in muscle-specific protein expression in differentiating MPCs despite normal MyoD and elevated myogenin levels.
机译：肌肉少肌症缺乏的骨骼肌的再生，再生和/或肥大需要肌肉前体细胞（MPC）。在目前的调查中，我们已经解决了MPC分化中与年龄相关的变化问题。从成年和成年大鼠骨骼肌中分离出具有人造肌高度纯度（> 90％的MyoD和结蛋白阳性）的MPC，包括卫星细胞。与从3月龄大鼠分离的MPC相比，从32月龄大鼠骨骼肌分离的MPC表现出向肌管的分化减少，并显示出肌球蛋白重链（MHC）和肌酸激酶（CK-M）表达降低。 p27（Kip1）是一种细胞周期蛋白依赖性激酶抑制剂，已显示可增强培养物中的肌肉分化。在本文中，我们描述了我们的发现，即在区分32个月大大鼠骨骼肌的MPCs中，p27（Kip1）蛋白要比3个月大大鼠骨骼肌中的p27（Kip1）蛋白低。尽管在从32个月大的大鼠中分离出的MPC中，MHC和CK-M的表达降低了约50％，但MyoD的蛋白质含量没有差异，而肌生成素的蛋白质浓度却高出两倍。这些数据表明，在从肌少肌分离的MPC中，从细胞周期停滞到肌管形成的过渡过程中，细胞信号传导存在固有差异。此外，尽管MyoD正常且肌生成素水平升高，但在分化的MPC中，与肌肉相关的蛋白质表达却存在与年龄相关的下降。

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