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首页> 外文期刊>American Journal of Physiology >Impact of caveolin-1 knockout on NANC relaxation in circular muscles of the mouse small intestine compared with longitudinal muscles.
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Impact of caveolin-1 knockout on NANC relaxation in circular muscles of the mouse small intestine compared with longitudinal muscles.

机译：与纵向肌肉相比，caveolin-1基因敲除对小鼠小肠环形肌肉NANC松弛的影响。

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Recently, we showed that caveolin-1 (cav1) knockout mice (Cav1(-/-) mice) have impaired nitric oxide (NO) function in the longitudinal muscle (LM) layer of the small intestine. The defect was a reduced responsiveness of the muscles to NO compensated by an increase in the function of apamin-sensitive, nonadrenergic, noncholinergic (NANC) mediators. In the present study, we examined similarly the effects of cav1 knockout on the relaxation in circular muscle (CM) of the mouse small intestine. CM of Cav1(-/-) mice also showed defective NO function, but less than in LM, as well as more activation of apamin-sensitive NANC mediators. CM of Cav1(-/-) mice, like LM, lacked cav1 but retained small amounts of cav3 and caveolae in the outer CM layer. In addition, we also examined the effects of a soluble guanylate cyclase inhibitor, 1H-[1,2,4]oxadiazolo-[4,3-alpha]quinazolin-1-one (ODQ), on electric field stimulation (EFS)-mediated relaxation in both LM and CM. ODQ had an effect similar to the block of NO synthesis. Moreover, we compared the actions of two NO donors in the LM and CM of control and Cav1(-/-) mice. Similar to LM, CM of Cav1(-/-) mice showed a reduced responsiveness to the NO donors sodium nitroprusside and S-nitroso-N-acetyl penicillamine. However, both ODQ and apamin blocked the inhibitory effects of the NO donors in LM, whereas apamin had no effect in CM. In conclusion, cav1 knockout affects NO function in both LM and CM, but its effects in CM differ significantly.
机译：最近，我们表明，caveolin-1（cav1）敲除小鼠（Cav1（-/-）小鼠）在小肠的纵向肌肉（LM）层中一氧化氮（NO）功能受损。缺陷是通过增加对谷氨酰胺敏感的，非肾上腺素能的，非胆碱能（NANC）介质的功能补偿，肌肉对NO的反应性降低。在本研究中，我们类似地检查了cav1基因敲除对小鼠小肠环形肌（CM）松弛的影响。 Cav1（-/-）小鼠的CM也显示出有缺陷的NO功能，但比LM中的NO功能低，并且对apamin敏感的NANC介质的激活也更多。 Cav1（-/-）小鼠的CM像LM一样，缺少cav1，但是在CM的外层保留了少量的cav3和小窝。此外，我们还研究了可溶性鸟苷酸环化酶抑制剂1H- [1,2,4]恶二唑-[4,3-α-喹唑啉-1-酮（ODQ）对电场刺激（EFS）-介导的LM和CM松弛。 ODQ具有类似于NO合成阻滞的作用。此外，我们比较了两个NO供体在对照组和Cav1（-/-）小鼠的LM和CM中的作用。与LM相似，Cav1（-/-）小鼠的CM对NO供体硝普钠和S-亚硝基-N-乙酰青霉素的反应性降低。然而，ODQ和阿帕明都阻断了NO供体对LM的抑制作用，而阿帕明对CM没有作用。总之，cav1基因敲除会影响LM和CM的NO功能，但其在CM中的作用差异很大。

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