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首页> 外文期刊>American Journal of Physiology >Dietary protein and lactose increase translation initiation factor activation and tissue protein synthesis in neonatal pigs.
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Dietary protein and lactose increase translation initiation factor activation and tissue protein synthesis in neonatal pigs.

机译：日粮蛋白和乳糖可提高新生猪的翻译起始因子激活和组织蛋白合成。

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Protein synthesis and eukaryotic initiation factor (eIF) activation are increased in muscle and liver of pigs parenterally infused with amino acids and insulin. To examine the effects of enteral protein and carbohydrate on protein synthesis, pigs (n = 42, 1.7 kg body wt) were fed isocaloric milk diets containing three levels of protein (5, 15, and 25 g x kg body wt(-1) x day(-1)) and two levels of lactose (low = 11 and high = 23 g x kg body wt(-1) x day(-1)) from 1 to 6 days of age. On day 7, pigs were gavage fed after 4-h food deprivation, and tissue protein synthesis rates and biomarkers of mRNA translation were assessed. Piglet growth and protein synthesis rates in muscle and liver increased with dietary protein and plateaued at 15 g x kg body wt(-1) x day(-1) (P < 0.001). Growth tended to be greater in high-lactose-fed pigs (P = 0.07). Plasma insulin was lowest in pigs fed 5 g x kg body wt(-1) x day(-1) protein (P < 0.0001). Plasma branched-chain amino acids increased as protein intake increased (P< 0.0001). Muscle (P < 0.001) and liver (P < or = 0.001) ribosomal protein S6 kinase-1 and eIF4E-binding protein phosphorylation increased with protein intake and plateaued at 15 g x kg body wt(-1) x day(-1). The results indicate that growth and protein synthesis rates in neonatal pigs are influenced by dietary protein and lactose intake and might be mediated by plasma amino acids and insulin levels. However, feeding protein well above the piglet's requirement does not further stimulate the activation of translation initiation or protein synthesis in skeletal muscle and liver.
机译：肠胃外注射氨基酸和胰岛素的猪的肌肉和肝脏中的蛋白质合成和真核起始因子（eIF）激活增加。为了检查肠内蛋白质和碳水化合物对蛋白质合成的影响，给猪（n = 42，1.7公斤体重）喂饲含三种蛋白质水平（5、15和25 gx公斤体重（-1）x第（-1）天）和从1至6天龄的两个水平的乳糖（低= 11和高= 23 gx kg体重（-1）x天（-1））。在第7天的食物被剥夺后的第4天，对猪进行管饲，并评估组织蛋白合成率和mRNA翻译的生物标志物。仔猪肌肉和肝脏中的生长和蛋白质合成速率随日粮蛋白质的增加而增加，并在15 g x kg体重wt（-1）x天（-1）时达到稳定状态（P <0.001）。高乳糖喂养的猪的生长趋向于更大（P = 0.07）。饲喂5 g x kg体重（-1）x day（-1）蛋白的猪的血浆胰岛素最低（P <0.0001）。血浆支链氨基酸随着蛋白质摄入的增加而增加（P <0.0001）。肌肉（P <0.001）和肝脏（P <或= 0.001）核糖体蛋白S6激酶-1和eIF4E结合蛋白的磷酸化随蛋白摄入量的增加而增加，并稳定在15 g x kg体重wt（-1）x天（-1）。结果表明，新生猪的生长和蛋白质合成速率受日粮蛋白质和乳糖摄入量的影响，并可能受血浆氨基酸和胰岛素水平的介导。然而，饲喂蛋白质远高于仔猪的需求量并不能进一步刺激骨骼肌和肝脏中翻译起始或蛋白质合成的激活。

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