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首页> 外文期刊>American Journal of Physiology >Effect of extracellular osmolality on cell volume and resting metabolism in mammalian skeletal muscle.
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Effect of extracellular osmolality on cell volume and resting metabolism in mammalian skeletal muscle.

机译：细胞外渗透压对哺乳动物骨骼肌细胞体积和静息代谢的影响。

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摘要

The purpose of the present investigation was to establish an in vitro mammalian skeletal muscle model to study acute alterations in resting skeletal muscle cell volume. Isolated, whole muscles [soleus and extensor digitorum longus (EDL)] were dissected from Long-Evans rats and incubated for 60 min in Sigma medium 199 (1 g of resting tension, bubbled with 95% O(2)-5% O(2), 30 +/- 2 degrees C, and pH 7.4). Medium osmolality was altered to simulate hyposmotic (190 +/- 10 mmol/kg) or hyperosmotic conditions (400 +/- 10 mmol/kg), whereas an isosmotic condition (290 +/- 10 mmol/kg) served as a control. After incubation, relative water content of the muscle decreased with hyperosmotic and increased with hyposmotic condition in both muscle types (P < 0.05). The cross-sectional area of soleus type I and type II fibers increased (P < 0.05) in hyposmotic, whereas hyperosmotic exposure led to no detectable changes. The EDL type II fiber area decreased in the hyperosmotic condition and increased after hyposmotic exposure, whereas no change was observed in EDL type I fibers. Furthermore, exposure to the hyperosmotic condition in both muscle types resulted in decreased muscle ATP and phosphocreatine (P < 0.05) contents and increased creatine and lactate contents (P < 0.05) compared with control and hyposmotic conditions. This isolated skeletal muscle model proved viable and demonstrated that altering extracellular osmolality could cause acute alterations in muscle water content and resting muscle metabolism.
机译：本研究的目的是建立体外哺乳动物骨骼肌模型，以研究静止骨骼肌细胞体积的急性变化。从Long-Evans大鼠中分离出完整的肌肉[比目鱼肌和趾长伸肌（EDL）]，并在Sigma 199培养基（1 g静息张力，以95％O（2）-5％O（ 2），30 +/- 2摄氏度和pH 7.4）。改变中等重量克分子渗透压浓度以模拟低渗（190 +/- 10 mmol / kg）或高渗条件（400 +/- 10 mmol / kg），而等渗条件（290 +/- 10 mmol / kg）作为对照。孵育后，两种肌肉类型的肌肉的相对含水量均随着高渗而降低，并随着低渗条件而增加（P <0.05）。低渗性比目鱼I型和II型纤维的横截面积增加（P <0.05），而高渗暴露导致未检测到变化。在高渗条件下，EDL II型纤维面积减少，而在低渗暴露后，EDL II型纤维面积增加，而在EDL I型纤维中未观察到变化。此外，与对照和低渗状态相比，两种肌肉都暴露于高渗状态导致肌肉ATP和磷酸肌酸含量降低（P <0.05），而肌酸和乳酸含量增加（P <0.05）。这种孤立的骨骼肌模型被证明是可行的，并证明改变细胞渗透压可以引起肌肉含水量和静息肌肉代谢的急性改变。

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