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首页> 外文期刊>American Journal of Physiology >Short-term blackcurrant extract consumption modulates exercise-induced oxidative stress and lipopolysaccharide-stimulated inflammatory responses
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Short-term blackcurrant extract consumption modulates exercise-induced oxidative stress and lipopolysaccharide-stimulated inflammatory responses

机译：短期服用黑加仑提取物可调节运动引起的氧化应激和脂多糖刺激的炎症反应

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Exercise-induced oxidative stress is instrumental in achieving the health benefits from regular exercise. Therefore, inappropriate use of fruit-derived products (commonly applied as prophalytic antioxidants) may counteract the positive effects of exercise. Using human exercise and cellular models we found that 1) blackcurrant supplementation suppressed exercise-induced oxidative stress, e.g., plasma carbonyls (0.9 +- 0.1 vs. 0.6 +- 0.1 nmol/mg protein, placebo vs. blackcurrant), and 2) preincubation of THP-1 cells with an anthocyanin-rich blackcurrant extract inhibited LPS-stimulated cytokine secretion [TNF-alpha (16,453 +- 322 vs. 10,941 +- 82 pg/ml, control vs. extract, P < 0.05) and IL-6 (476 +- 14 vs. 326 +- 32 pg/ml, control vs. extract, P < 0.05)] and NF-kappaB activation. In addition to its antioxidant and anti-inflammatory properties, we found that postexercise plasma collected after blackcurrant supplementation enhanced the differential temporal LPS-stimulated inflammatory response in THP-1 cells, resulting in an early suppression of TNF-alpha (1,741 +- 32 vs. 1,312 +- 42 pg/ml, placebo vs. blackcurrant, P < 0.05) and IL-6 (44 +- 5 vs. 36 +- 3 pg/ml, placebo vs. blackcurrant, P < 0.05) secretion after 24 h. Furthermore, by using an oxidative stress cell model, we found that preincubation of THP-1 cells with hydrogen peroxide (H_2O_2) prior to extract exposure caused a greater suppression of LPS-stimulated cytokine secretion after 24 h, which was not evident when cells were simultaneously incubated with H_2O_2 and the extract. In summary, our findings support the concept that consumption of blackcurrant anthocyanins alleviate oxidative stress, and may, if given at the appropriate amount and time, complement the ability of exercise to enhance immune responsiveness to potential pathogens.
机译：运动引起的氧化应激有助于通过定期运动获得健康益处。因此，不适当使用水果衍生产品（通常用作促分解型抗氧化剂）可能抵消锻炼的积极作用。使用人类运动和细胞模型，我们发现1）黑加仑补充剂可抑制运动诱导的氧化应激，例如血浆羰基化合物（0.9±-0.1 vs. 0.6 +-0.1 nmol / mg蛋白，安慰剂与黑加仑），以及2）预孵育富含花青素的黑加仑提取物对THP-1细胞的抑制作用抑制了LPS刺激的细胞因子分泌[TNF-α（16,453 +-322 vs. 10,941 +-82 pg / ml，对照vs.提取物，P <0.05）和IL-6 （476±14对326±32 pg / ml，对照对提取物，P ＜0.05）]和NF-κB活化。除了其抗氧化和抗炎特性外，我们发现补充黑醋栗后收集的运动后血浆可增强THP-1细胞中时空LPS刺激的差异性炎症反应，从而导致TNF-α的早期抑制（1,741 +-32 vs 。24小时后，分泌了1,312 +-42 pg / ml，安慰剂与黑加仑，P <0.05）和IL-6（44 +-5 vs. 36 +-3 pg / ml，安慰剂与黑加仑，P <0.05）分泌。此外，通过使用氧化应激细胞模型，我们发现提取物暴露之前，THP-1细胞与过氧化氢（H_2O_2）的预温育会在24小时后对LPS刺激的细胞因子分泌产生更大的抑制作用，而当同时与H_2O_2和提取物一起孵育。总而言之，我们的发现支持以下概念：食用黑加仑花青素可以缓解氧化应激，如果以适当的量和时间给予，可以补充运动的能力，以增强对潜在病原体的免疫应答。

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