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首页> 外文期刊>American Journal of Physiology >Effect of sex differences on human MEF2 regulation during endurance exercise
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Effect of sex differences on human MEF2 regulation during endurance exercise

机译：性别差异对耐力运动中人MEF2调节的影响

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First published November 27, 2007; doi:10.n52/ajpendo.00403.2007.-Women exhibit an enhanced capability for lipid metabolism during endurance exercise compared with men. The underlying regulatory mechanisms behind this sex-related difference are not well understood but may comprise signaling through a myocyte enhancer factor 2 (MEF2) regulatory pathway. The primary purpose of this study, therefore, was to investigate the protein signaling of MEF2 regulatory pathway components at rest and during 90 min of bicycling exercise at 60% Vo_(2peak) in healthy, moderately trained men (n = 8) and women (73 = 9) to elucidate the potential role of these proteins in substrate utilization during exercise. A secondary purpose was to screen for mRNA expression of MEF2 isoforms and myogenic regulatory factor (MRF) family members of transcription factors at rest and during exercise. Muscle biopsies were obtained before and immediately after exercise. Nuclear AMP-activated protein kinase-a (aAMPK) Thr172 (P < 0.001), histone deacetylase 5 (HDAC5) Ser498 (P < 0.001), and MEF2 Thr (P < 0.01) phosphorylation increased with exercise. No significant sex differences were observed at rest or during exercise. At rest, no significant sex differences were observed in mRNA expression of the measured transcription factors. mRNA for transcription factors MyoD, myogenin, MRF4, MEF2A, MEF2C, MEF2D, and peroxisome proliferator-activated receptor-y coactivator Ialpha (PGCla) were significantly upregulated by exercise. Of these, MEF2A mRNA increased 25% specifically in women (P < 0.05), whereas MEF2D mRNA tended to increase hi men (P - 0.11). Although minor sex differences in mRNA expression were observed, the main finding of the present study was the implication of a joint signaling action of AMPK, HDAC5, and PGCla on MEF2 in the immediate regulatory response to endurance exercise. This signaling response was independent of sex.
机译：首次发布于2007年11月27日; doi：10.n52 / ajpendo.00403.2007.-与男性相比，女性在耐力运动中的脂质代谢能力增强。这种与性别相关的差异背后的潜在调节机制尚未得到很好的理解，但可能包括通过心肌细胞增强因子2（MEF2）调节途径的信号传导。因此，本研究的主要目的是研究健康，中度训练的男性（n = 8）和女性（60％Vo_（2peak），在静止和骑自行车运动90分钟期间MEF2调节途径成分的蛋白质信号传导， 73 = 9），以阐明这些蛋白质在运动过程中对底物利用的潜在作用。第二个目的是筛选静止和运动过程中MEF2亚型和转录因子的肌调节因子（MRF）家族成员的mRNA表达。运动前和运动后立即进行肌肉活检。核AMP活化蛋白激酶a（aAMPK）Thr172（P <0.001），组蛋白脱乙酰基酶5（HDAC5）Ser498（P <0.001）和MEF2 Thr（P <0.01）磷酸化随运动而增加。在休息或运动期间没有观察到明显的性别差异。静止时，在所测转录因子的mRNA表达中未观察到明显的性别差异。锻炼可显着上调转录因子MyoD，肌生成素，MRF4，MEF2A，MEF2C，MEF2D和过氧化物酶体增殖物激活的受体-γ共激活因子Ialpha（PGCla）的mRNA。其中，MEF2A mRNA在女性中特异性增加25％（P <0.05），而MEF2D mRNA在男性中倾向于增加（P-0.11）。尽管观察到mRNA表达中的微小性别差异，但是本研究的主要发现是在耐力运动的即时调节反应中，AMPK，HDAC5和PGCla的联合信号传导作用对MEF2具有影响。这种信号反应与性别无关。

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