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首页> 外文期刊>American Journal of Physiology >Effects of maternal genotype and diet on offspring glucose and fatty acid-sensing ventromedial hypothalamic nucleus neurons.
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Effects of maternal genotype and diet on offspring glucose and fatty acid-sensing ventromedial hypothalamic nucleus neurons.

机译：母体基因型和饮食对子代葡萄糖和脂肪酸敏感的腹内侧下丘脑核神经元的影响。

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Maternal obesity accentuates offspring obesity in dams bred to develop diet-induced obesity (DIO) on a 31% fat, high-sucrose, high-energy (HE) diet but has no effect on offspring of diet-resistant (DR) dams. Also, only DIO dams become obese when they and DR dams are fed HE diet throughout gestation and lactation. We assessed glucose and oleic acid (OA) sensitivity of dissociated ventromedial hypothalamic nucleus (VMN) neurons from 3- to 4-wk old offspring of DIO and DR dams fed chow or HE diet using fura-2 calcium imaging to monitor intracellular calcium fluctuations as an index of neuronal activity. Offspring of DIO dams fed chow had approximately 2-fold more glucose-inhibited (GI) neurons than did DR offspring. This difference was eliminated in offspring of DIO dams fed HE diet. At 2.5 mM glucose, offspring of chow-fed DIO dams had more GI neurons that were either excited or inhibited by OA than did DR offspring. Maternal HE diet intake generally increased the percentage of neurons that were excited and decreased the percentage that were inhibited by OA in both DIO and DR offspring. However, this effect was more pronounced in DIO offspring. These data, as well as concentration-dependent differences in OA sensitivity, suggest that genotype, maternal obesity, and dietary content can all affect the sensitivity of offspring VMN neurons to glucose and long-chain fatty acids. Such altered sensitivities may underlie the propensity of DIO offspring to become obese when fed high-fat, high-sucrose diets.
机译：母体肥胖会加剧大坝中的后代肥胖，这些肥胖会以31％的脂肪，高蔗糖，高能量（HE）饮食发展为饮食诱导型肥胖（DIO），但对耐饮食性（DR）大坝的后代没有影响。同样，只有DIO大坝和DR大坝在整个妊娠和哺乳期接受HE饮食时才变得肥胖。我们使用呋喃2钙成像技术评估了饲喂食物或HE饮食的DIO和DR大坝的3至4周大后代的离体腹膜下丘脑核（VMN）神经元对葡萄糖和油酸（OA）的敏感性，并通过Fura-2钙成像来监测细胞内钙的波动神经活动指数。用DIO饲料喂养的DIO大坝的后代比DR后代的葡萄糖抑制（GI）神经元大约多2倍。用HE饮食喂养的DIO大坝的后代消除了这种差异。葡萄糖为2.5 mM时，与DR的后代相比，以食物喂养的DIO大坝的后代具有更多的被OA激发或抑制的GI神经元。母体HE饮食摄入通常会增加DIO和DR后代的兴奋神经元百分比，并降低被OA抑制的百分比。但是，这种效应在DIO后代中更为明显。这些数据以及OA敏感性的浓度依赖性差异表明，基因型，母体肥胖和饮食含量均可影响后代VMN神经元对葡萄糖和长链脂肪酸的敏感性。当进食高脂，高蔗糖日粮时，这种敏感性的改变可能是DIO后代倾向于肥胖的基础。

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