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首页> 外文期刊>American Journal of Physiology >Publishing flow cytometry data.
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Publishing flow cytometry data.

机译：发布流式细胞仪数据。

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摘要

Cellular measurements by flow cytometric analysis constitute an important step toward understanding individual attributes within a population of cells. Assessing individual cells within a population by protein expression using fluorescently labeled antibodies and other fluorescent probes can identify cellular patterns. The technology for accurately identifying subtle changes in protein expression within a population of cells using a vast array of technology has resulted in controversy and questions regarding reproducibility, which can be explained at least in part by the absence of standard methods to facilitate comparison of flow cytometric data. The complexity of technological advancements and the need for improvements in biological resolution results in the generation of complex data that demands the use of minimum standards for their publication. Herein we present a summarized view for the inclusion of consistent flow cytometric experimental information as supplemental data. Four major points, experimental and sample information, data acquisition, analysis, and presentation are emphasized. Together, these guidelines will facilitate the review and publication of flow cytometry data that provide an accurate foundation for ongoing studies with this evolving technology.
机译：通过流式细胞术分析进行细胞测量是迈向了解细胞群内各个属性的重要一步。使用荧光标记的抗体和其他荧光探针通过蛋白质表达评估种群中的单个细胞可以识别细胞模式。使用大量技术来准确识别细胞群中蛋白质表达的细微变化的技术引起了争议和有关可重复性的问题，这至少可以部分地通过缺乏促进流式细胞术比较的标准方法来解释数据。技术进步的复杂性以及对生物分辨率的改进需求导致生成复杂的数据，这些数据需要使用最低标准来发布。在这里，我们为包含一致的流式细胞仪实验信息作为补充数据提供了一个概述视图。强调了四个主要方面，即实验和样本信息，数据采集，分析和表示。这些指南将共同促进流式细胞仪数据的审查和发布，从而为正在进行的这项技术研究提供准确的基础。

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