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首页> 外文期刊>Analytical chemistry >Integrated label-free protein detection and separation in real time using confined surface plasmon resonance imaging
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Integrated label-free protein detection and separation in real time using confined surface plasmon resonance imaging

机译：使用受限的表面等离振子共振成像实时进行集成的无标记蛋白质检测和分离

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摘要

We demonstrate a label-free protein detection and separation technology for real-time monitoring of proteins in microanofluidic channels, confined surface plasmon resonance imaging (confined-SPRi). This was achieved by fabricating ultrathin fluidic channels (500 nm high, 500 mu m wide) directly on top of a specialized SPRi sensor surface. In this way, SPRi is uniquely used to detect proteins deep into the fluidic channel while maintaining high lateral accuracy of separated products. The channel fluid and proteins were driven electrokinetically under an external electric field. For this to occur, the metallic SPR sensor (46 nm of Au on 2 nm of Cr) was segmented into an array of squares (each 200 mu m x 200 mu m in size and spaced 8 mu m apart) and coated with 30 nm of CYTOP polymer. In this work, we track label-free protein separation in real time through a simple cross-junction fluidic device with an 8-mm separation channel length under 30 V/cm electric field strength.
机译：我们演示了无标签的蛋白质检测和分离技术，用于实时监测微/纳流体通道中的蛋白质，受限的表面等离振子共振成像（受限SPRi）。这是通过在专用SPRi传感器表面的顶部直接制造超薄流体通道（高500 nm，宽500μm）来实现的。这样，SPRi可以独特地用于检测进入流体通道深处的蛋白质，同时保持分离产物的高横向精度。通道流体和蛋白质在外部电场下被电动驱动。为此，将金属SPR传感器（46 nm的Au在2 nm的Cr上）切成正方形阵列（每个200μmx 200μm的尺寸，彼此间隔8μm），并涂上30 nm的CYTOP聚合物。在这项工作中，我们通过在30 V / cm电场强度下具有8 mm分离通道长度的简单交叉结流体装置实时跟踪无标记蛋白的分离。

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