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首页> 外文期刊>Analytical chemistry >Surface-Enhanced Raman Spectroscopic Detection of a Bacteria Biomarker Using Gold Nanoparticle Immobilized Substrates
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Surface-Enhanced Raman Spectroscopic Detection of a Bacteria Biomarker Using Gold Nanoparticle Immobilized Substrates

机译：使用金纳米粒子固定化基质的细菌生物标记物的表面增强拉曼光谱检测

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The development of ultrasensitive and rapid methods for the detection of dipicolinic acid (DPA), a biomarker for bacterial spores including Bacillus anthracis, is increasingly important. This paper reports the results of an investigation of surface enhanced Raman spectroscopy (SERS) based ultrasensitive detection of DPA using a gold nanoparticle/polyvinylpyrrolidone/gold substrate (AuNPs/PVP/Au). The strong SERS effect of this substrate exploits the particle-particle and particle-substrate plasmonic coupling, which is optimized by manipulating the diameter of the nanoparticles (50-70 nm). The correlation between the SERS intensity of the diagnostic band and the DPA concentration (0.1 ppb to 100 ppm) was shown to exhibit two linear regions, i.e., the low- (<0.01 ppm) and high-concentration (>1 ppm) regions, with an intermediate region in between. The presence of a linear relationship in the low-concentration region was observed for the first time in SERS detection of DPA. A detection limit of 0.1 ppb was obtained from the substrates with 60 nm sized Au NPs, which is, to our knowledge, the lowest detection limit reported for DPA using this type of SERS substrate. This finding was also supported by the estimated enhancement factor (approx 10~(6)) and a large adsorption equilibrium constant for the low-concentration region (1.7 X 10~(7) M~(-1)). The adsorption characteristics of DPA on the SERS substrates were analyzed in terms of monolayer and multilayer adsorption isotherms to gain insights into the correlation between the SERS intensity and the DPA concentration. The observed transition from the low- to high-concentration linear regions was found to correspond to the transition from a monolayer to multilayer adsorption isotherm, which was in agreement with the estimated minimum DPA concentration for a monolayer coverage (approx0.01 ppm).
机译：开发用于检测包括炭疽芽孢杆菌在内的细菌孢子的生物标志物二吡啶甲酸（DPA）的超灵敏，快速方法的重要性日益增加。本文报道了使用金纳米粒子/聚乙烯吡咯烷酮/金底物（AuNPs / PVP / Au）对DPA进行超灵敏检测的表面增强拉曼光谱（SERS）研究结果。这种基质的强大SERS效应利用了颗粒-颗粒和颗粒-基质的等离子体耦合，这通过控制纳米颗粒的直径（50-70 nm）得以优化。诊断谱带的SERS强度与DPA浓度（0.1 ppb至100 ppm）之间的相关性显示出两个线性区域，即低（<0.01 ppm）和高浓度（> 1 ppm）区域，中间有一个中间区域。在DERS的SERS检测中首次观察到低浓度区域存在线性关系。从具有60 nm尺寸的Au NP的底物获得0.1 ppb的检测限，据我们所知，这是使用这种类型的SERS底物报道的DPA的最低检测限。估计的增强因子（约10〜（6））和低浓度区域的大吸附平衡常数（1.7 X 10〜（7）M〜（-1））也支持了这一发现。根据单层和多层吸附等温线分析了DPA在SERS基底上的吸附特性，以深入了解SERS强度与DPA浓度之间的相关性。发现从低浓度到高浓度线性区域的转变对应于从单层到多层吸附等温线的转变，这与估计的单层覆盖率的最小DPA浓度一致（约0.01 ppm）。

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