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首页> 外文期刊>Analytical chemistry >~(13)C Isotope-Labeled Metabolomes Allowing for Improved Compound Annotation and Relative Quantification in Liquid Chromatography-Mass Spectrometry-based Metabolomic Research
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~(13)C Isotope-Labeled Metabolomes Allowing for Improved Compound Annotation and Relative Quantification in Liquid Chromatography-Mass Spectrometry-based Metabolomic Research

机译：〜（13）C同位素标记的代谢物，可在基于液相色谱-质谱的代谢组学研究中改善化合物的注释和相对定量

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摘要

Metabolomics is rapidly becoming an integral part of many life science studies ranging from disease diagnostics to systems biology. However, a number of problems such as the discrimination of biological from non-biological signals, efficient compound annotation, and reliable quantification are still not satisfactorily solved in untargeted LC-MS-based metabolomics research. Extending our previous work on direct infusion-based metabolomics, we here describe a ~(13)C isotope labeling strategy in combination with an Ultra Performance Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry-based approach (UPLC-FTICR MS) which provides a technological platform offering solutions to a number of the above-mentioned problems. We further demonstrate that the use of a fully labeled metabolome is not only beneficial for high end mass spectrometers, such as that used in this study but also provides a considerable improvement to every other mass spectrometry-based metabolomic platform.
机译：从疾病诊断到系统生物学，代谢组学正迅速成为许多生命科学研究的组成部分。但是，在基于LC-MS的非靶向代谢组学研究中，仍然无法令人满意地解决许多问题，例如从非生物信号中区分生物学信号，有效的化合物注释和可靠的定量分析。作为对基于直接输注的代谢组学工作的扩展，我们在此描述〜（13）C同位素标记策略，结合基于超高效液相色谱傅里叶变换离子回旋共振质谱的方法（UPLC-FTICR MS），技术平台，可以解决上述许多问题。我们进一步证明，使用完全标记的代谢组不仅对高端质谱仪（如本研究中使用的质谱仪）有益，而且还对其他所有基于质谱的代谢组学平台提供了相当大的改进。

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