• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Analytical chemistry >Coupling Surface-Enhanced Resonance Raman Scattering and Electronic Tongue as Characterization Tools to Investigate Biological Membrane Mimetic Systems
【24h】

Coupling Surface-Enhanced Resonance Raman Scattering and Electronic Tongue as Characterization Tools to Investigate Biological Membrane Mimetic Systems

机译：耦合表面增强共振拉曼散射和电子舌作为表征工具来研究生物膜模拟系统

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The surface-enhanced Raman scattering (SERS) effect and sensor and biosensor analyses are widely applied to investigate drug-biomolecule interactions or to detect trace amount of analytes. In this work, surface-enhanced resonance Raman scattering (SERRS) and an electronic tongue system using impedance spectroscopy were brought together, combining sensitivity and structural level information. Taking advantage of the use of layer-by-layer (LbL) films of phospholipids as biological membrane mimetic systems, cardiolipin (CLP) and dipalmitoyl phosphatidyl glycerol (DPPG) were applied as transducers onto Pt interdigitated electrodes forming an array of sensing units. This e-tongue system was able to detect the phenothiazine methylene blue (MB) below nanomolar concentrations. SERRS was applied to investigate the MB molecular arrangement (monomers or aggregates) when in contact with the phospholipids at trace levels of concentration. The key point was the adsorption of Ag nanoparticles (AgNPs) within the phospholipid LbL films. This approach did not compromise the e-tongue performance and allowed the recording of in situ SERRS spectra for the LbL films after immersion into MB aqueous solutions. The detection of MB through SERRS gave similar results to those reported in the literature but now with an unprecedented sensitivity.
机译：表面增强拉曼散射（SERS）效应以及传感器和生物传感器分析已广泛应用于研究药物与生物分子的相互作用或检测痕量分析物。在这项工作中，结合了灵敏度和结构水平信息，将表面增强共振拉曼散射（SERRS）和使用阻抗谱的电子舌系统结合在一起。利用磷脂的逐层（LbL）膜作为生物膜模拟系统，心磷脂（CLP）和二棕榈酰磷脂酰甘油（DPPG）作为换能器应用于形成传感单元阵列的Pt叉指电极上。该电子舌系统能够检测到低于纳摩尔浓度的吩噻嗪亚甲基蓝（MB）。 SERRS用于研究以微量浓度与磷脂接触时的MB分子排列（单体或聚集体）。关键是磷脂LbL膜中银纳米颗粒（AgNPs）的吸附。这种方法不会影响电子舌的性能，并允许在浸入MB水溶液后记录LbL膜的原位SERRS光谱。通过SERRS对MB的检测与文献报道的结果相似，但现在具有空前的灵敏度。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号