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首页> 外文期刊>Analytical chemistry >Tunnel Frit: A Nonmetallic In-Capillary Frit for Nanoflow Ultra High-Performance Liquid Chromatography-Mass SpectrometryApplications
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Tunnel Frit: A Nonmetallic In-Capillary Frit for Nanoflow Ultra High-Performance Liquid Chromatography-Mass SpectrometryApplications

机译：隧道玻璃料：一种用于纳流超高效液相色谱-质谱法的非金属毛细管内玻璃料

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摘要

In this study, an easy method to fabricate a durable in-capillary frit was developed for use in nanoflow liquid chromatography (nanoLC). A small orifice was tunneled into the sol-gel frit during the polymerization process resulting in the simple fabrication of a tunnel frit. A short packing tunnel frit column (2 cm, C_(18) particles) was able to sustain over 10000 psi continuous liquid flow for 10 days without observation of particle loss, and back pressure variation was less than 5percent. The tunnel frit was successfully applied to the fabrication of nanoflow ultra high-performance liquid chromatography (nano-UHPLC) trap and analytical columns. In the analysis of tryptic peptides, the tunnel frit trap and analytical columns were demonstrated to have high separation efficiency and sensitivity. In analysis of phosphopeptides, the use of the nonmetallic tunnel frit column showed better sensitivity than the metallic frit column. This design can facilitate the preparation of nano-HPLC and nano-UHPLC columns and the packing material can easily be refilled when the column is severely contaminated or clogged.
机译：在这项研究中，开发了一种用于制造耐用的毛细管内熔块的简便方法，用于纳流液相色谱（nanoLC）。在聚合过程中，一个小孔被隧穿到溶胶-凝胶熔块中，导致隧道熔块的简单制造。短填料隧道熔块色谱柱（2 cm，C_（18）颗粒）能够在超过10000 psi的连续液体流量下保持10天，而没有观察到颗粒损失，并且背压变化小于5％。隧道熔块已成功应用于纳流超高效液相色谱（nano-UHPLC）捕集阱和分析柱的制造。在胰蛋白酶肽的分析中，隧道玻璃料捕集阱和分析柱被证明具有很高的分离效率和灵敏度。在分析磷酸肽时，使用非金属隧道熔块色谱柱显示出比金属熔块色谱柱更好的灵敏度。这种设计可以促进纳米HPLC和纳米UHPLC色谱柱的制备，并且当色谱柱受到严重污染或堵塞时，填充材料可以轻松地重新填充。

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