Laser Electrospray Mass Spectrometry Minimizes Ion Suppression Facilitating Quantitative Mass Spectral Response for Multicomponent Mixtures of Proteins

Johnny J. Perez; Paul M. Flanigan; Santosh Karki; Robert J. Levis

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Laser Electrospray Mass Spectrometry Minimizes Ion Suppression Facilitating Quantitative Mass Spectral Response for Multicomponent Mixtures of Proteins

机译：激光电喷雾质谱可最大程度地减少离子抑制，有利于蛋白质多组分混合物的定量质谱响应

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A comparison of the mass spectral response for myoglobin, cytochrome c, and lysozyme is presented for laser electrospray mass spectrometry (LEMS) and electrospray ionization--mass spectrometry (ESI-MS). Analysis of multi-component protein solutions using nonresonant femtosecond (fs) laser vaporization with electrospray postionization mass spectrometry exhibited significantly reduced ion suppression effects in comparison with conventional ESI analysis, enabling quantitative measurements over 4 orders of magnitude in concentration. No significant charge reduction was observed in the LEMS experiment while the ESI measurement revealed charge reduction for myoglobin and cytochrome c as a function of increasing protein concentration. Conventional ESI--MS of each analyte from a multicomponent solution reveals that the ion signal detected for myoglobin and cytochrome c reaches a plateau and then begins to decrease with increasing protein concentration preventing quantitative analysis. The ESI mass spectral response for lysozyme from the mixture initially decreased, before increasing, with increasing multicomponent solution concentration.

机译：针对激光电喷雾质谱（LEMS）和电喷雾电离质谱（ESI-MS），对肌红蛋白，细胞色素c和溶菌酶的质谱响应进行了比较。与传统的ESI分析相比，使用非共振飞秒（fs）激光汽化和电喷雾后离子化质谱分析多组分蛋白质溶液显示出显着降低的离子抑制效果，从而可以定量测量4个数量级以上的浓度。在LEMS实验中未观察到明显的电荷减少，而ESI测量显示，肌红蛋白和细胞色素c的电荷减少是蛋白质浓度增加的函数。通过多组分溶液对每种分析物进行的常规ESI-MS分析表明，检测到的肌红蛋白和细胞色素c的离子信号达到平稳状态，然后随着蛋白质浓度的增加而开始下降，从而阻止了定量分析。混合物中溶菌酶的ESI质谱响应最初随着多组分溶液浓度的增加而降低，然后升高。

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《Analytical chemistry》 |2013年第14期|共7页
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Johnny J. Perez; Paul M. Flanigan; Santosh Karki; Robert J. Levis;
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1. Laser Electrospray Mass Spectrometry Minimizes Ion Suppression Facilitating Quantitative Mass Spectral Response for Multicomponent Mixtures of Proteins [J] . Johnny J. Perez, Paul M. Flanigan, Santosh Karki, Analytical chemistry . 2013,第14期

机译：激光电喷雾质谱可最大程度地减少离子抑制，有利于蛋白质多组分混合物的定量质谱响应
2. Determination of loperamide in Mdr1a/1b knock-out mouse brain tissue using matrix-assisted laser desorption/ionization mass spectrometry and comparison with quantitative electrospray-triple quadrupole mass spectrometry analysis [J] . ShinY.G., DongT., ChouB., Archives of pharmacal research . 2011,第11期

机译：基质辅助激光解吸/电离质谱法测定Mdr1a / 1b敲除小鼠脑组织中的洛哌丁胺，并与定量电喷雾三重四极杆质谱法进行比较
3. Determination of loperamide in Mdr1a/1b knock-out mouse brain tissue using matrix-assisted laser desorption/ionization mass spectrometry and comparison with quantitative electrospray-triple quadrupole mass spectrometry analysis [J] . Young G. Shin, Teresa Dong, Bilin Chou, Archives of Pharmacal Research . 2011,第11期

机译：基质辅助激光解吸/电离质谱法测定Mdr1a / 1b敲除小鼠脑组织中的洛哌丁胺，并与定量电喷雾三重四极杆质谱法进行比较
4. On-line HPLC with H/D Exchange in Liquid Electrospray-Assisted Laser Desorption Ionization Mass Spectrometry for The Analysis of Protein Mixture [C] . Yi-Tzu Cho, Jingyueh Jeng, Jentaie Shiea American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：在线HPLC具有H / D型液体电喷雾辅助激光解吸电离质谱法分析蛋白质混合物
5. Identification and Analysis of Proteins Using Matrix Assisted Laser Desorption Ionization and Electrospray Ionization Mass Spectrometry [D] . Quebbemann, Neil Robert. 2020

机译：用基质辅助激光解吸电离和电喷雾电离质谱法鉴定和分析蛋白质
6. Electrospray-assisted Laser Desorption Ionization Mass Spectrometry (ELDI-MS) with an Infrared Laser for Characterizing Peptides and Proteins [O] . Ivory X. Peng, Rachel R. Ogorzalek Loo, Eli Margalith, -1

机译：电喷雾辅助的激光解吸电离质谱（ELDI-ms）与红外线激光为表征肽和蛋白质
7. Application of an integrated matrix-assisted laser desorption/ionization time-of-flight, electrospray ionization mass spectrometry and tandem mass spectrometry approach to characterizing complex polyol mixtures [O] . Chen Rui, Tseng Amy M., Uhing Mary, 2001

机译：集成基质辅助激光解吸/电离飞行时间，电喷雾电离质谱和串联质谱方法在表征复杂多元醇混合物中的应用
8. Electrospray ionization mass spectrometry and tandem mass spectrometry for mixture analysis of proteins and oligopeptides. [R] . C. G. Edmonds K. J. Light R. D. Smith J. A. Loo R. R. O. Loo 1991

机译：用于蛋白质和寡肽混合物分析的电喷雾电离质谱和串联质谱。

Laser Electrospray Mass Spectrometry Minimizes Ion Suppression Facilitating Quantitative Mass Spectral Response for Multicomponent Mixtures of Proteins

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