...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Analytical chemistry >Universal Surface-Enhanced Raman Scattering Amplification Detector for Ultrasensitive Detection of Multiple Target Analytes
【24h】

Universal Surface-Enhanced Raman Scattering Amplification Detector for Ultrasensitive Detection of Multiple Target Analytes

机译：通用表面增强拉曼散射放大检测器，用于多目标分析物的超灵敏检测

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Up to now, the successful fabrication of efficient hot-spot substrates for surface-enhanced Raman scattering (SERS) remains an unsolved problem. To address this issue, we describe herein a universal aptamer-based SERS biodetection approach that uses a single-stranded DNA as a universal trigger (UT) to induce SERS-active hot-spot formation, allowing, in turn, detection of a broad range of targets. More specifically, interaction between the aptamer probe and its target perturbs a triple-helix aptamer/UT structure in a manner that activates a hybridization chain reaction (HCR) among three short DNA building blocks that self-assemble into a long DNA polymer. The SERS-active hot-spots are formed by conjugating 4-aminobenzenethiol (4-ABT)-encoded gold nanoparticles with the DNA polymer through a specific Au-S bond. As proof-of-principle, we used this approach to quantify multiple target analytes, including thrombin, adenosine, and CEM cancer cells, achieving lowest limit of detection values of 18 pM, 1.5 nM, and 10 cells/mL, respectively. As a universal SERS detector, this prototype can be applied to many other target analytes through the use of suitable DNA-functional partners, thus inspiring new designs and applications of SERS for bioanalysis.
机译：迄今为止，成功制造用于表面增强拉曼散射（SERS）的高效热点衬底的问题仍未解决。为了解决这个问题，我们在这里描述了一种基于适体的通用SERS生物检测方法，该方法使用单链DNA作为通用触发（UT）来诱导SERS活性热点的形成，进而允许在宽范围内进行检测目标。更具体地，适体探针与其靶标之间的相互作用以激活自组装成长DNA聚合物的三个短DNA构件之间的杂交链反应（HCR）的方式扰动三螺旋适体/ UT结构。 SERS活性热点是通过特定的Au-S键将4-氨基苯硫醇（4-ABT）编码的金纳米颗粒与DNA聚合物缀合而形成的。作为原理的证明，我们使用了这种方法来量化多个目标分析物，包括凝血酶，腺苷和CEM癌细胞，分别达到18 pM，1.5 nM和10细胞/ mL的最低检测限。作为通用的SERS检测器，该原型可以通过使用合适的DNA功能伙伴应用于其他许多目标分析物，从而激发了SERS的新设计和生物分析应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号